Behavioral Testing Battery
Objective: Assessment of antidepressant-like effects, general locomotor activity, anxiety-like behaviors, and social memory in optogenetically stimulated mice
This is a Behavioral Testing Battery protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 2 materials. Extracted from a 2010 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Chronic social defeat stress induction • Viral vector injection and channel rhodopsin 2 expression • Optogenetic stimulation of medial prefrontal cortex
Primary readouts
- Antidepressant-like effects
- General locomotor activity
- Anxiety-like behaviors
- Social memory
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Chronic social defeat stress induction
Mice subjected to chronic social defeat stress as a mouse model of depression
Note: Results in distinction between susceptible animals (strong depressive-like phenotype) and resilient animals (escape deleterious consequences)
View evidence from paper
“Mice subjected to chronic social defeat stress, a mouse model of depression”
Viral vector injection and channel rhodopsin 2 expression
Viral vectors used to overexpress channel rhodopsin 2 in mouse medial prefrontal cortex
Note: Allows for optogenetic control of mPFC neuronal activity
View evidence from paper
“viral vectors to overexpress channel rhodopsin 2 (a light-activated cation channel) in mouse mPFC”
Optogenetic stimulation of medial prefrontal cortex
Light-activated stimulation of mPFC to drive burst patterns of cortical firing in vivo
Note: Applied to susceptible animals expressing strong depressive-like phenotype
View evidence from paper
“optogenetically drive burst patterns of cortical firing in vivo and examine the behavioral consequences”
Behavioral testing battery
Assessment of antidepressant-like effects, general locomotor activity, anxiety-like behaviors, and social memory
Note: Conducted following optogenetic stimulation
View evidence from paper
“optogenetic stimulation of mPFC exerted potent antidepressant-like effects, without affecting general locomotor activity, anxiety-like behaviors, or social memory”