Behavioral Testing
behavioralmouseC57BL/6
Objective: Behavioral testing of wild type and heterozygous Fus mutant mice to assess behavioral phenotypes
Protocol Steps
1
Animal housing and maintenance
Mice were housed in the central animal facility with a regular 12-hour light and dark cycle (light on at 7:00 am) under constant temperature and humidity conditions
21 ± 1°C
Note: Humidity maintained at 60%. Standard laboratory rodent food and water available ad libitum
View evidence from paper
“housed in the central animal facility of the Faculty of Medicine of Strasbourg, with a regular 12-h light and dark cycle (light on at 7:00 am) under constant conditions (21 ± 1°C; 60% humidity)”
2
Genotyping
Mice were genotyped by PCR of genomic DNA from tail biopsies
Note: Genotyping method described previously in reference 68
View evidence from paper
“Mice were genotyped by PCR of genomic DNA from tail biopsies as described previously”
3
Behavioral testing
Behavioral tests were conducted on 10- to 22-month-old male littermates of each genotype (Fus+/+, FusΔNLS/+, and Fus+/-) on a pure genetic background (C57BL/6)
Note: Tests conducted during the light phase of the light/dark cycle
View evidence from paper
“10- to 22-month-old male littermates of each genotype (Fus+/+, FusΔNLS/+ and Fus+/-) on a pure genetic background (C57/Bl6) were subjected to behavioral tests and molecular analyses. Behavioral test were done during the light phase of their light/dark cycle”