Behavioral Testing
Objective: Behavioral testing of wild type and heterozygous Fus mutant mice to assess behavioral phenotypes
Protocol Steps
Animal housing and maintenance
Mice were housed in the central animal facility with a regular 12-hour light and dark cycle (light on at 7:00 am) under constant temperature and humidity conditions
Note: Humidity maintained at 60%. Standard laboratory rodent food and water available ad libitum
View evidence from paper
“housed in the central animal facility of the Faculty of Medicine of Strasbourg, with a regular 12-h light and dark cycle (light on at 7:00 am) under constant conditions (21 ± 1°C; 60% humidity)”
Genotyping
Mice were genotyped by PCR of genomic DNA from tail biopsies
Note: Genotyping method described previously in reference 68
View evidence from paper
“Mice were genotyped by PCR of genomic DNA from tail biopsies as described previously”
Behavioral testing
Behavioral tests were conducted on 10- to 22-month-old male littermates of each genotype (Fus+/+, FusΔNLS/+, and Fus+/-) on a pure genetic background (C57BL/6)
Note: Tests conducted during the light phase of the light/dark cycle
View evidence from paper
“10- to 22-month-old male littermates of each genotype (Fus+/+, FusΔNLS/+ and Fus+/-) on a pure genetic background (C57/Bl6) were subjected to behavioral tests and molecular analyses. Behavioral test were done during the light phase of their light/dark cycle”