Behavioral Testing
Objective: Assess the potential of MENPs to wirelessly modulate neuronal activity via magnetoelectric response to applied magnetic field and measure effects on mouse behavior
Protocol Steps
Magnetic field application to nanoparticles
Apply magnetic field to MENPs and control nanoparticles. Experimental design includes application of large DC magnetic field overlaid with smaller AC magnetic field for magnetoelectric effect measurement, with AC and DC magnetic field application alone used as experimental controls.
Note: Magnetoelectric effect expected only when applying large DC magnetic field overlaid with smaller AC magnetic field
View evidence from paper
“applying a magnetic field to the MENPs (and control nanoparticles) and measuring (i) their output electric signaling, (ii) their ability to modulate neuronal cell activity in culture, (iii) their ability to modulate brain activity in mice, and (iv) the effect of this modulation on mouse behavior”
Measure nanoparticle output electric signaling
Measure the output electric signaling from MENPs in response to applied magnetic field
View evidence from paper
“measuring (i) their output electric signaling”
Assess neuronal modulation in culture
Evaluate the ability of MENPs to modulate neuronal cell activity in culture
View evidence from paper
“their ability to modulate neuronal cell activity in culture”
Assess brain activity modulation in mice
Evaluate the ability of MENPs to modulate brain activity in mice
View evidence from paper
“their ability to modulate brain activity in mice”
Behavioral testing
Measure the effect of neuronal modulation on mouse behavior. Testing conducted with experimenter and data analyst blind to animal identity.
Note: Experimenter and data analyst blinded to animal identity
View evidence from paper
“Animal behavioral testing was done with the experimenter and data analyst blind to animal identity”