Behavioral Testing
behavioralmousePV-Cre/NR1f/f and littermate controls (NR1f/f)
Objective: Assess phenotypic differences in awake mice through behavioral testing
Protocol Steps
1
Animal generation and breeding
Generate PV-Cre/NR1f/f mice by crossing PV-Cre mice with mice carrying floxed NR1 alleles
Not specifiedNot specified
Note: Littermate male controls (NR1f/f) were used for behavioral testing comparisons
View evidence from paper
“We generated PV-Cre/NR1f/f mice by crossing of PV-Cre mice with mice carrying 'floxed' NR1 alleles”
2
Subject selection for behavioral testing
Select male PV-Cre/NR1f/f mice and littermate male controls (NR1f/f) for awake behavioral tests
Not specifiedNot specified
Note: Only male mice were used for awake electrophysiology and behavior tests
View evidence from paper
“In awake electrophysiology and behavior tests, male PV-Cre/NR1f/f mice and littermate male controls (NR1f/f) were used”
3
Conduct behavioral tests
Perform behavioral tests on awake mice to assess phenotypic differences between genotypes
Not specifiedNot specified
Note: Specific behavioral tests are not detailed in the provided methods text
View evidence from paper
“In awake electrophysiology and behavior tests, male PV-Cre/NR1f/f mice and littermate male controls (NR1f/f) were used”