Complement<i>C3</i>-Deficient Mice Fail to Display Age-Related Hippocampal Decline

Qiaoqiao Shi, Kenneth J. Colodner, Sarah B. Matousek, Katherine Merry, Soyon Hong et al.

Journal of Neuroscience • 2015

DOI PMC

Behavioral Testing

behavioralmouseC57BL/6J

Objective: Assessment of learning, memory, cognition, and anxiety levels in mice at different ages to examine behavioral changes and synaptic health

This is a Behavioral Testing protocol using mouse as the model organism. The procedure involves 2 procedural steps. Extracted from a 2015 paper published in Journal of Neuroscience.

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Replication Essentials

Model and subjects

mouse • C57BL/6J • male • postnatal day 30, 4 months, and 16 months

Study window

Estimated timing pending

Core workflow

Subject Selection and Grouping • Behavioral Testing

Primary readouts

Synapse loss (region-specific and age-dependent)
Neuron loss in hippocampal CA3
Long-term potentiation (LTP)
Cognition performance

Key equipment and reagents

Procurement Snapshot

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Protocol Steps

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Subject Selection and Grouping

C3-deficient male mice (C3 KO) were compared with age-, strain-, and gender-matched C57BL/6J wild-type (WT) control mice

Note: Testing conducted at three age timepoints: postnatal day 30, 4 months, and 16 months

View evidence from paper

“C3 -deficient male mice ( C3 KO) compared with age-, strain-, and gender-matched C57BL/6J (wild-type, WT) control mice at postnatal day 30, 4 months, and 16 months of age”