Black and White Box Test
Objective: Assessment of anxiety-like behaviors in mice using black and white box apparatus during evaluation of memory and behavioral deficits
This is a Black and White Box Test protocol using mouse as the model organism. The procedure involves 4 procedural steps, 4 equipment items, 1 materials. Extracted from a 2014 paper published in Brain.
Model and subjects
mouse • C57BL6/J • unknown • Not specified • Not specified
Study window
~2 week study window
Core workflow
Surgical implantation of ventricular catheters and osmotic pumps • Continuous infusion of cerebrospinal fluid • Black and white box test for anxiety assessment
Primary readouts
- Anxiety-like behaviors in black and white box test
- Memory deficits (novel object recognition, V-maze)
- Anhedonic behaviors (sucrose preference)
- Depressive-like behaviors (tail suspension, forced swimming)
Key equipment and reagents
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Protocol Steps
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Surgical implantation of ventricular catheters and osmotic pumps
C57BL6/J mice underwent placement of ventricular catheters connected to osmotic pumps for continuous infusion delivery
Note: Catheters connected to osmotic pumps delivering continuous infusion
View evidence from paper
“C57BL6/J mice underwent placement of ventricular catheters connected to osmotic pumps”
Continuous infusion of cerebrospinal fluid
Osmotic pumps delivered continuous infusion of either patients' cerebrospinal fluid or control cerebrospinal fluid
Note: Flow rate maintained at 0.25 µl/h
View evidence from paper
“osmotic pumps that delivered a continuous infusion of patients' or control cerebrospinal fluid (flow rate 0.25 µl/h, 14 days)”
Black and white box test for anxiety assessment
Mice were tested in black and white box apparatus during and after the infusion period to assess anxiety-like behaviors
Note: Test performed as part of standardized behavioral assessment battery
View evidence from paper
“During and after the infusion period standardized tests were applied, including tasks to assess memory (novel object recognition in open field and V-maze paradigms), anhedonic behaviours (sucrose preference test), depressive-like behaviours (tail suspension, forced swimming tests), anxiety (black and white, elevated plus maze tests)”
Tissue collection and analysis
Animals were sacrificed at specified timepoints for examination of brain-bound antibodies and NMDAR effects
Note: Sacrifice timepoints: Days 5, 13, 18, 26, and 46
View evidence from paper
“Animals sacrificed at Days 5, 13, 18, 26 and 46 were examined for brain-bound antibodies and the antibody effects on total and synaptic NMDAR clusters and protein concentration using confocal microscopy and immunoblot analysis”