Body Weight and Food Intake Monitoring

“Eighty-four male C57BL/6JOlaHsd mice (Harlan Laboratories, Horst, The Netherlands) were individually housed under controlled conditions (temperature 21°C, 12 h/12 h light–dark cycle, 55 ± 15 % humidity), with ad libitum access to food and water. At arrival, the mice were 9 weeks of age.”

“During the first 5 days of a 3-week adaptation period, mice were fed a standard Harlan chow diet”

“followed by a standardised semi-synthetic normal-fat diet [NF, 10 energy% (en%) fat] with the same dietary constituents as the intervention high-fat diet (HF, 40 en%) in which carbohydrates were substituted with fats”

“At the start of the 12-week intervention period, mice were stratified based on body weight over 7 groups ( n = 12)”

“One group of mice continued on NF, while the other six groups of mice received HF with or without supplementation of different flavonoids (HF + flavonoids). Flavonoids were added in equimolar amounts to HF (0.01 mol/kg diet)”

“Body weight and food intake were monitored weekly”

“Body weight and food intake were monitored weekly”

“Faeces were collected in weeks 11 and 12”

“At the end of the intervention, all mice were fasted for 2–4 h during the light phase”

“anesthetised by inhalation of 5 % isoflurane using O 2 as a carrier”

“Blood was sampled via orbital extraction in collect serum tubes (Greiner Bio-one, Longwood, USA) and stored at −80 °C after obtaining serum”

“After blood collection, mice were killed by cervical dislocation, and liver, epididymal and mesenteric white adipose tissues (epiWAT and mesWAT, resp.) were dissected, weighted and snap frozen in liquid nitrogen and stored at −80 °C”