Body Weight and Locomotor Activity Monitoring
Objective: Assessment of LPS-induced sickness behavior through measurement of body weight loss, decreased motor activity, and reduced food intake
Gather these items before starting the experiment. Check off items as you prepare.
Materials3
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Protocol Steps
Baseline measurements
Establish baseline body weight, motor activity, and food intake prior to LPS administration
Note: These measurements serve as baseline for comparison of sickness behavior
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”
Ketamine administration
Administer ketamine at 6 mg/kg intraperitoneally immediately before LPS administration
Note: Route of administration is intraperitoneal
View evidence from paper
“a low dose of ketamine (6 mg/kg, intraperitoneally) immediately before administration of LPS”
LPS administration
Administer LPS at 0.83 mg/kg intraperitoneally to induce sickness behavior
Note: Route of administration is intraperitoneal
View evidence from paper
“administration of LPS (0.83 mg/kg, intraperitoneally) in C57Bl/6 J mice”
Monitor body weight
Measure body weight loss as indicator of sickness behavior
Note: Body weight loss is measured as one of three sickness behavior indicators
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”
Monitor motor activity
Measure decreased motor activity as indicator of sickness behavior
Note: Decreased motor activity is measured as one of three sickness behavior indicators
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”
Monitor food intake
Measure reduced food intake as indicator of sickness behavior
Note: Reduced food intake is measured as one of three sickness behavior indicators
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”