Body Weight and Locomotor Activity Monitoring
Objective: Assessment of LPS-induced sickness behavior through measurement of body weight loss, decreased motor activity, and reduced food intake
This is a Body Weight and Locomotor Activity Monitoring protocol using mouse as the model organism. The procedure involves 6 procedural steps, 3 materials. Extracted from a 2013 paper published in Neuropsychopharmacology.
Model and subjects
mouse • C57BL/6J • unknown • Not specified • Not specified
Study window
~72 hours hands-on
Core workflow
Baseline measurements • Ketamine administration • LPS administration
Primary readouts
- Body weight loss
- Decreased motor activity
- Reduced food intake
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Baseline measurements
Establish baseline body weight, motor activity, and food intake prior to LPS administration
Note: These measurements serve as baseline for comparison of sickness behavior
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”
Ketamine administration
Administer ketamine at 6 mg/kg intraperitoneally immediately before LPS administration
Note: Route of administration is intraperitoneal
View evidence from paper
“a low dose of ketamine (6 mg/kg, intraperitoneally) immediately before administration of LPS”
LPS administration
Administer LPS at 0.83 mg/kg intraperitoneally to induce sickness behavior
Note: Route of administration is intraperitoneal
View evidence from paper
“administration of LPS (0.83 mg/kg, intraperitoneally) in C57Bl/6 J mice”
Monitor body weight
Measure body weight loss as indicator of sickness behavior
Note: Body weight loss is measured as one of three sickness behavior indicators
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”
Monitor motor activity
Measure decreased motor activity as indicator of sickness behavior
Note: Decreased motor activity is measured as one of three sickness behavior indicators
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”
Monitor food intake
Measure reduced food intake as indicator of sickness behavior
Note: Reduced food intake is measured as one of three sickness behavior indicators
View evidence from paper
“LPS-induced sickness measured by body weight loss, decreased motor activity, and reduced food intake”