Body Weight Monitoring
Objective: Track body weight changes and monitor diabetes onset in creatine-supplemented versus control transgenic mice (R6/2 line) to assess neuroprotective effects of dietary creatine supplementation
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Equipment2
Materials1
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Protocol Steps
Establish treatment groups
Divide R6/2 transgenic mice into creatine-supplemented and control groups
Note: Study compares creatine-supplemented R6/2 mice versus control R6/2 mice
View evidence from paper
“creatine-supplemented R6/2 mice, whereas the onset of diabetes was markedly delayed”
Administer dietary creatine supplementation
Provide creatine supplementation to treatment group through diet
Note: Control group receives standard diet without creatine supplementation
View evidence from paper
“Dietary creatine supplementation significantly improved survival, slowed the development of brain atrophy”
Monitor body weight
Regularly measure and track body weight changes in both creatine-supplemented and control mice
Note: Body weight is a key outcome measure for assessing disease progression
View evidence from paper
“Body weight and motor performance on the rotarod test were significantly improved in creatine-supplemented R6/2 mice”
Monitor diabetes onset
Track and record the development and timing of diabetes in both treatment and control groups
Note: Diabetes onset is delayed in creatine-supplemented mice compared to controls
View evidence from paper
“the onset of diabetes was markedly delayed”
Perform rotarod motor performance testing
Assess motor coordination and balance using rotarod apparatus in both groups
Note: Motor performance is significantly improved in creatine-supplemented mice
View evidence from paper
“Body weight and motor performance on the rotarod test were significantly improved in creatine-supplemented R6/2 mice”