Experiments/Bronchial Hyperreactivity Assessment

Source Paper

House dust mite allergen induces asthma via Toll-like receptor 4 triggering of airway structural cells

Hamida Hammad, Marcello Chieppa, Frederic Perros, Monique A Willart, Ronald N Germain et al.

Nature Medicine • 2009

DOI PMC

Bronchial Hyperreactivity Assessment

behavioralmouseNot specified in provided text

Objective: Measurement of bronchial hyperreactivity as a marker of asthma features in response to HDM (house dust mite) allergen challenge

This is a Bronchial Hyperreactivity Assessment protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2009 paper published in Nature Medicine.

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Replication Essentials

Model and subjects

mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text

Study window

Estimated timing pending

Core workflow

HDM allergen challenge • Bronchial hyperreactivity assessment • TLR4 antagonist treatment

Primary readouts

Bronchial hyperreactivity
Allergic airway inflammation
DC activation in the lung
Effector T helper responses

Key equipment and reagents

Bronchial hyperreactivity measurement apparatusHouse dust mite (HDM) allergen

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Protocol Steps

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HDM allergen challenge

Mice were exposed to house dust mite allergen via inhalation to induce allergic airway inflammation and asthma-like features

Not specified in provided textNot specified in provided text

Note: This challenge was used to establish HDM-driven allergic airway inflammation

View evidence from paper

“TLR4 expression on radioresistant lung structural cells is required and sufficient for DC activation in the lung and for priming of effector T helper responses to HDM”