Bronchial Hyperreactivity Assessment
Objective: Measurement of bronchial hyperreactivity as a marker of asthma features in response to HDM (house dust mite) allergen challenge
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Equipment1
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Protocol Steps
HDM allergen challenge
Mice were exposed to house dust mite allergen via inhalation to induce allergic airway inflammation and asthma-like features
Note: This challenge was used to establish HDM-driven allergic airway inflammation
View evidence from paper
“TLR4 expression on radioresistant lung structural cells is required and sufficient for DC activation in the lung and for priming of effector T helper responses to HDM”
Bronchial hyperreactivity assessment
Measurement of bronchial hyperreactivity as a salient feature of asthma in response to HDM challenge
Note: Bronchial hyperreactivity was one of the key asthma features evaluated
View evidence from paper
“inhalation of a TLR4 antagonist to target exposed epithelial cells suppressed the salient features of asthma including bronchial hyperreactivity”
TLR4 antagonist treatment
Administration of TLR4 antagonist via inhalation to target exposed epithelial cells
Note: Treatment was designed to suppress asthma features by targeting epithelial cells
View evidence from paper
“inhalation of a TLR4 antagonist to target exposed epithelial cells suppressed the salient features of asthma including bronchial hyperreactivity”