Experiments/CatWalk Gait Analysis

Source Paper

Transplantation of Induced Pluripotent Stem Cell-Derived Neural Stem Cells Mediate Functional Recovery Following Thoracic Spinal Cord Injury Through Remyelination of Axons

Ryan P. Salewski, Robert A. Mitchell, Lijun Li, Carl Shen, Maria Milekovskaia et al.

Stem Cells Translational Medicine • 2015

DOI PMC

CatWalk Gait Analysis

behavioralmouseWildtype and Shiverer mouse lines

Objective: Quantitative assessment of walking patterns and gait parameters following spinal cord injury treatment using CatWalk gait analysis

This is a CatWalk Gait Analysis protocol using mouse as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 2 materials. Extracted from a 2015 paper published in Stem Cells Translational Medicine.

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Replication Essentials

Model and subjects

mouse • Wildtype and Shiverer mouse lines • unknown • Not specified • Not specified

Study window

Estimated timing pending

Core workflow

Spinal cord injury induction • Subacute intraspinal transplantation • CatWalk gait analysis assessment

Primary readouts

Neurobehavioral function via CatWalk gait analysis
Neurobehavioral function via Basso Mouse Scale
Histological outcomes including graft integration and oligodendrocyte differentiation
Electrophysiological measures of axonal function via sucrose gap analysis

Key equipment and reagents

CatWalk Gait Analysis SystemInduced pluripotent stem cells (iPS cells)

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Protocol Steps

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Spinal cord injury induction

Thoracic spinal cord injury was induced in mice

Not specifiedNot specified

Note: Thoracic level injury model used

View evidence from paper

“following thoracic spinal cord injury (spinal cord injury)”

Subacute intraspinal transplantation

NSCs derived from wildtype (wt) and nonmyelinating Shiverer (shi) iPS cell lines were transplanted into the injured spinal cord

Subacute timepoint (not specified exactly)Not specified

Note: Two treatment groups: wt-iPS-NSCs and shi-iPS-NSCs, plus cell-free controls

View evidence from paper

“NSCs derived from wildtype (wt) and nonmyelinating Shiverer (shi) iPS cell lines were used following thoracic SCI with subacute intraspinal transplantation”