Source Paper
Transplantation of Induced Pluripotent Stem Cell-Derived Neural Stem Cells Mediate Functional Recovery Following Thoracic Spinal Cord Injury Through Remyelination of Axons
Ryan P. Salewski, Robert A. Mitchell, Lijun Li, Carl Shen, Maria Milekovskaia et al.
Stem Cells Translational Medicine • 2015
CatWalk Gait Analysis
Objective: Quantitative assessment of walking patterns and gait parameters following spinal cord injury treatment using CatWalk gait analysis
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Materials2
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Protocol Steps
Spinal cord injury induction
Thoracic spinal cord injury was induced in mice
Note: Thoracic level injury model used
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“following thoracic spinal cord injury (spinal cord injury)”
Subacute intraspinal transplantation
NSCs derived from wildtype (wt) and nonmyelinating Shiverer (shi) iPS cell lines were transplanted into the injured spinal cord
Note: Two treatment groups: wt-iPS-NSCs and shi-iPS-NSCs, plus cell-free controls
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“NSCs derived from wildtype (wt) and nonmyelinating Shiverer (shi) iPS cell lines were used following thoracic SCI with subacute intraspinal transplantation”
CatWalk gait analysis assessment
Quantitative assessment of walking patterns and gait parameters was performed using CatWalk system
Note: Part of neurobehavioral outcome assessment
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“neurobehavioral function (Basso Mouse Scale and CatWalk)”
Basso Mouse Scale assessment
Behavioral assessment of locomotor function using Basso Mouse Scale
Note: Complementary behavioral assessment to CatWalk
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“neurobehavioral function (Basso Mouse Scale and CatWalk)”
Histological analysis
Histological outcomes were analyzed to assess tissue response and graft integration
Note: Assessed graft integration and differentiation
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“Behavioral, histological, and electrophysiological outcomes were analyzed”
Electrophysiological assessment
Sucrose gap analysis was performed to measure axonal function
Note: Measured electrophysiological measures of axonal function
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“electrophysiological measures of axonal function (sucrose gap analysis)”