Chronic Corticosterone Treatment
Objective: To establish a mouse model of chronic stress through corticosterone administration and evaluate the effects on anxiety and depression-related behaviors
Gather these items before starting the experiment. Check off items as you prepare.
Materials1
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Protocol Steps
Chronic Corticosterone Administration
Administer corticosterone chronically to establish a mouse model of stress
Note: This creates the stress condition for testing the effects of increased adult neurogenesis
View evidence from paper
“mice treated chronically with corticosterone (CORT), a mouse model of stress”
Behavioral Testing Under Chronic Stress
Evaluate anxiety and depression-related behaviors in mice receiving chronic corticosterone treatment
Note: Testing measures whether increased adult neurogenesis reduces anxiety and depression-like behaviors
View evidence from paper
“increasing adult neurogenesis is sufficient to reduce anxiety and depression-related behaviors in mice treated chronically with corticosterone”
HPA Axis Assessment
Evaluate hypothalamic-pituitary-adrenal (HPA) axis regulation at baseline and following chronic CORT administration
Note: Determines whether increased neurogenesis affects HPA axis function
View evidence from paper
“no effect of increased adult hippocampal neurogenesis on hypothalamic–pituitary–adrenal (HPA) axis regulation, either at baseline or following chronic CORT administration”