Source Paper
Computational modeling reveals cognitive processes in simple rodent depression tests
Li Z, Lu T, Yan J, Zhang X, Li YF
Cell Rep Methods • 2025
Chronic Restraint Stress (CRS)
Objective: To induce chronic stress in mice through prolonged daily restraint for studying stress-related behavioral and neurobiological changes
This is a Chronic Restraint Stress (CRS) protocol using Mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Cell Rep Methods.
Model and subjects
Mouse • C57Bl/6 • Male • 8 weeks old at the start of experiments • Not specified
Study window
~8 hours hands-on
Core workflow
Daily restraint protocol • Duration of treatment • Post-session handling
Primary readouts
- Stress-induced behavioral changes
- Neurobiological alterations from chronic stress exposure
Key equipment and reagents
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Protocol Steps
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Daily restraint protocol
Restrain mice in tubes with limited movement for 8 hours daily from 10:00 a.m. to 6:00 p.m.
View evidence from paper
“mice were restrained in tubes with limited movement for 8 h daily (10:00 a.m.–6:00 p.m.)”
Duration of treatment
Continue daily restraint sessions for 21 consecutive days
View evidence from paper
“over 21 consecutive days”
Post-session handling
After each restraint session, release mice and return them to their home cages
View evidence from paper
“After each restraint session, the mice were released and returned to their home cages”
Control group
Keep control mice in their home cages without exposure to stress
View evidence from paper
“Control mice were kept in their home cages without exposure to stress”