Stress‐Induced Activation of Prolactin‐NR4A1‐Midkine Axis Exacerbates Skin Inflammation

Li Z, Quan H, Liu W, Chen J, Chu M et al.

Adv Sci (Weinh) • 2025

Chronic Restraint Stress (CRS)

behavioralMouseNot specified

Objective: To induce sustained anxiety and depression-like behaviors in mice to study the impact of chronic psychological stress on skin inflammation

Materials & Equipment Checklist

2 items

Gather these items before starting the experiment. Check off items as you prepare.

Imiquimod (IMQ)

Agomelatine

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Apply chronic restraint stress to mice using a validated murine model

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“we employed a chronic restraint stress (CRS) mouse model”

Apply topical imiquimod to induce skin inflammation in both stressed and control mice

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“induced skin inflammation with topical imiquimod (IMQ)”

Create four experimental groups: Con+Vehicle, CRS+Vehicle, Con+IMQ, CRS+IMQ

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“four experimental groups: Con+Vehicle, CRS+Vehicle, Con+IMQ, CRS+IMQ”