Source Paper
Computational modeling reveals cognitive processes in simple rodent depression tests
Li Z, Lu T, Yan J, Zhang X, Li YF
Cell Rep Methods • 2025
Chronic Restraint Stress
Objective: To induce chronic stress in mice through prolonged daily restraint for behavioral and neurobiological studies
This is a Chronic Restraint Stress protocol using Mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Cell Rep Methods.
Model and subjects
Mouse • C57Bl/6 • Male • 8 weeks old at the start of experiments • Not specified
Study window
~8 hours hands-on
Core workflow
Acclimatization period • Daily restraint stress application • Duration of stress protocol
Primary readouts
- Behavioral changes in response to chronic stress
- Neurobiological alterations following chronic restraint stress
Key equipment and reagents
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Protocol Steps
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Acclimatization period
Allow mice to acclimate to housing conditions for one week before beginning stress protocol
View evidence from paper
“Behavioral experiments commenced after one week of acclimatization”
Daily restraint stress application
Restrain mice in tubes with limited movement for 8 hours daily from 10:00 a.m. to 6:00 p.m.
View evidence from paper
“mice were restrained in tubes with limited movement for 8 h daily (10:00 a.m.–6:00 p.m.)”
Duration of stress protocol
Continue daily restraint stress for 21 consecutive days
View evidence from paper
“over 21 consecutive days”
Post-restraint handling
Release mice from restraint tubes and return them to their home cages after each session
View evidence from paper
“After each restraint session, the mice were released and returned to their home cages”
Control group management
Keep control mice in their home cages without exposure to restraint stress
View evidence from paper
“Control mice were kept in their home cages without exposure to stress”