Source Paper
Mitochondrial CISD1 Modulates Microglial Metabolic Reprogramming to Drive Stress Susceptibility in Mice
Dong W, Liu D, Fu S, Zhang J, Chen X et al.
Adv Sci (Weinh) • 2025
Chronic Social Defeat Stress
Objective: To induce chronic social defeat stress to create a depressive-like model in mice, categorizing them into susceptible and resilient groups based on behavioral responses
Protocol Steps
Chronic Social Defeat Stress Administration
Mice are subjected to chronic social defeat stress protocol
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“CSDS is a classic method for constructing depressive‐like model mice”
Behavioral Categorization
Based on core characteristics of depression (anhedonia and social avoidance), mice treated with CSDS are categorized into two groups: susceptible and resilient mice
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“mice treated with CSDS were categorized into two groups: susceptible and resilient mice”
Social Interaction Assessment
Susceptible mice show significantly reduced social interaction ratio and time in the interaction zone, along with increased time in the corner zone
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“susceptible mice showed a significantly reduced social interaction ratio and time in the interaction zone, along with increased time in the corner zone”
Sucrose Preference Test
Test reveals that susceptible mice exhibit reduced sucrose preference percentage compared with control group
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“susceptible mice exhibited a reduced sucrose preference percentage, whereas resilient mice showed no significant change”
Forced Swim Test and Tail Suspension Test
Susceptible mice exhibit significantly longer immobility time compared with control group
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“susceptible mice exhibited significantly longer immobility time, whereas no significant changes were observed in resilient mice”