Chronic Stress Exposure
Objective: To investigate how paternal stress exposure during puberty or adulthood affects offspring HPA stress axis regulation and to examine potential epigenetic mechanisms of germ cell transmission through sperm microRNA content changes
This is a Chronic Stress Exposure protocol using mouse as the model organism. The procedure involves 3 procedural steps. Extracted from a 2013 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • male • Stress exposure during puberty or adulthood; breeding age not specified • Not specified in provided text
Study window
~6 week study window
Core workflow
Chronic Stress Exposure - Puberty Group • Chronic Stress Exposure - Adulthood Group • Breeding
Primary readouts
- HPA stress axis responsivity in offspring
- Gene expression patterns in offspring stress-regulating brain regions (paraventricular nucleus and bed nucleus of stria terminalis)
- Glucocorticoid-responsive gene expression in the PVN
- Sperm microRNA content changes
Key equipment and reagents
Verified items
0
Direct vendor links
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Protocol Steps
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Chronic Stress Exposure - Puberty Group
Expose male mice to 6 weeks of chronic stress throughout puberty period before breeding
Note: Stress exposure occurs during puberty developmental period
View evidence from paper
“exposed mice to 6 weeks of chronic stress before breeding. As epidemiological studies support variation in paternal germ cell susceptibility to reprogramming across the lifespan, male stress exposure occurred either throughout puberty or in adulthood”
Chronic Stress Exposure - Adulthood Group
Expose male mice to 6 weeks of chronic stress during adulthood before breeding
Note: Stress exposure occurs during adulthood developmental period
View evidence from paper
“exposed mice to 6 weeks of chronic stress before breeding. As epidemiological studies support variation in paternal germ cell susceptibility to reprogramming across the lifespan, male stress exposure occurred either throughout puberty or in adulthood”
Breeding
Breed male mice following completion of 6-week chronic stress exposure period
Note: Breeding occurs after stress exposure is complete
View evidence from paper
“exposed mice to 6 weeks of chronic stress before breeding”