Chronic Stress Exposure
behavioralmouseNot specified in provided text
Objective: To investigate how paternal stress exposure during puberty or adulthood affects offspring HPA stress axis regulation and to examine potential epigenetic mechanisms of germ cell transmission through sperm microRNA content changes
Protocol Steps
1
Chronic Stress Exposure - Puberty Group
Expose male mice to 6 weeks of chronic stress throughout puberty period before breeding
6 weeksNot specified
Note: Stress exposure occurs during puberty developmental period
View evidence from paper
“exposed mice to 6 weeks of chronic stress before breeding. As epidemiological studies support variation in paternal germ cell susceptibility to reprogramming across the lifespan, male stress exposure occurred either throughout puberty or in adulthood”
2
Chronic Stress Exposure - Adulthood Group
Expose male mice to 6 weeks of chronic stress during adulthood before breeding
6 weeksNot specified
Note: Stress exposure occurs during adulthood developmental period
View evidence from paper
“exposed mice to 6 weeks of chronic stress before breeding. As epidemiological studies support variation in paternal germ cell susceptibility to reprogramming across the lifespan, male stress exposure occurred either throughout puberty or in adulthood”
3
Breeding
Breed male mice following completion of 6-week chronic stress exposure period
Not specifiedNot specified
Note: Breeding occurs after stress exposure is complete
View evidence from paper
“exposed mice to 6 weeks of chronic stress before breeding”