Experiments/Circadian Behavioral Analysis

Source Paper

Circadian control of brain glymphatic and lymphatic fluid flow

Lauren M. Hablitz, Virginia Plá, Michael Giannetto, Hanna S. Vinitsky, Frederik Filip Stæger et al.

Nature Communications • 2020

DOI PMC

Circadian Behavioral Analysis

behavioralmouseC57BL/6 and AQP4 KO (backcrossed to C57BL/6 for 20+ generations)

Objective: Analyze circadian behavioral patterns to determine free-running period and confirm behavioral rhythmicity in mice using activity monitoring and periodogram analysis

This is a Circadian Behavioral Analysis protocol using mouse as the model organism. The procedure involves 6 procedural steps, 2 equipment items, 1 materials. Extracted from a 2020 paper published in Nature Communications.

Workflow actionsRequest QuoteOpen Source Paper

Jump to Vendor Comparison

Overview Experimental Context Protocol Vendor Comparison Materials Checklist Evidence / Verification Related Experiments

Replication Essentials

Model and subjects

mouse • C57BL/6 and AQP4 KO (backcrossed to C57BL/6 for 20+ generations) • unknown • 3-5 months • 25-30g

Study window

~1.4 week study window

Core workflow

Animal acquisition and breeding • Housing setup • Continuous activity monitoring

Primary readouts

Free-running period of circadian activity
Behavioral rhythmicity confirmation
Activity patterns in 5-minute bins
Circadian phase (activity onset timing)

Key equipment and reagents

Comprehensive Lab Animal Monitoring System (CLAMS)Animal housing cages

Before You Start

Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.

Confirm first

Verify the animal model, intervention setup, and collection timepoints against the source paper.
Check that every direct vendor link matches the exact specification your lab plans to run.

Use the page like this

Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.

Jump to vendor comparison Jump to checklist Jump to methods evidence

Protocol Steps

Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.

Animal acquisition and breeding

Acquire male and female C57BL/6 mice from Charles River Laboratories in equal numbers. Breed AQP4 KO mice in University of Rochester vivarium and backcross to C57BL/6 for 20+ generations.

Not specifiedNot specified

Note: Equal numbers of males and females used to control for sex differences; minimum of five mice per group

View evidence from paper

“Male and female C57BL/6 mice (aged 3–5 months, weight between 25 g and 30 g) were acquired from Charles River Laboratories (Wilmington, MA) in equal numbers for each experimental group”

Housing setup

House mice either in a 12:12 light/dark cycle or under constant light with ad libitum access to food and water. For constant light experiments, house animals two per cage.

Not specified18-26 degrees Celsius

Note: Housing two per cage in constant light reduces risk of hypothermia; maintain humidity at 30-70%

View evidence from paper

“Mice were group-housed either in a 12:12 light/dark cycle or under constant light with ad libitum access to food and water”

Continuous activity monitoring

Monitor activity continuously using the Comprehensive Lab Animal Monitoring System (Columbus Instruments) with data recorded in 5-minute bins.

At least 10 days of activity data collectionNot specified

Note: Data collection must span minimum 10 days for accurate periodogram analysis

View evidence from paper

“Activity was monitored continuously in 5 min bins via the Comprehensive Lab Animal Monitoring System (Columbus Instruments)”