Source Paper
Kappa opioid receptor antagonism and prodynorphin gene disruption block stress-induced behavioral responses.
Jay P McLaughlin, Monica Marton-Popovici, Charles Chavkin
PubMed • 2003
Cocaine-Conditioned Place Preference
Objective: Assessment of cocaine reward by measuring preference for a drug-paired chamber in mice exposed to forced swim stress or control conditions, and to test whether stress-induced dynorphin release mediates stress-induced potentiation of cocaine reward
This is a Cocaine-Conditioned Place Preference protocol using mouse as the model organism. The procedure involves 5 procedural steps, 3 equipment items, 2 materials. Extracted from a 2003 paper published in PubMed.
Model and subjects
mouse • C57Bl/6 • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Forced Swim Stress Exposure • Measurement of Stress-Induced Analgesia • nor-BNI Pretreatment (Experimental Group)
Primary readouts
- Stress-induced immobility response during forced swim test
- Tail withdrawal latency (measure of stress-induced analgesia)
- Cocaine-conditioned place preference (time spent in drug-paired chamber)
- Potentiation of cocaine CPP in stressed versus unstressed mice
Key equipment and reagents
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Protocol Steps
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Forced Swim Stress Exposure
C57Bl/6 mice subjected to repeated forced swim testing using a modified Porsolt procedure
Note: This produces characteristic stress-induced immobility response and stress-induced analgesia
View evidence from paper
“C57Bl/6 mice subjected to repeated forced swim testing (FST) using a modified Porsolt procedure at 30°C showed a characteristic stress-induced immobility response”
Measurement of Stress-Induced Analgesia
Tail withdrawal latency assay performed to measure stress-induced analgesia
Note: Used to assess analgesic response to stress
View evidence from paper
“stress-induced analgesia observed with a tail withdrawal latency assay”
nor-BNI Pretreatment (Experimental Group)
Pretreatment with κ opioid receptor antagonist nor-binaltorphimine administered intraperitoneally
Note: Administered to test whether κ opioid receptor activation mediates stress responses
View evidence from paper
“Pretreatment with the κ opioid receptor antagonist nor-binaltorphimine (nor-BNI; 10 mg/kg, i.p.)”
Cocaine Conditioning
Mice conditioned with cocaine via subcutaneous injection
Note: Cocaine dose 15 mg/kg administered subcutaneously during conditioning phase
View evidence from paper
“FST-exposed mice conditioned with cocaine (15 mg/kg, s.c.)”
Conditioned Place Preference Testing
Assessment of preference for the drug-paired chamber in stressed versus unstressed mice
Note: Measures time spent in cocaine-paired chamber compared to control chamber
View evidence from paper
“FST-exposed mice conditioned with cocaine (15 mg/kg, s.c.) showed significant potentiation of place preference for the drug-paired chamber”