Source Paper
Adding Insult to Injury: Cochlear Nerve Degeneration after “Temporary” Noise-Induced Hearing Loss
Sharon G. Kujawa, M. Charles Liberman
Journal of Neuroscience • 2009
Cochlear Functional Assays
Objective: Assessment of cochlear function and threshold sensitivity changes in response to noise exposure, including evaluation of afferent nerve terminal loss and cochlear nerve degeneration
This is a Cochlear Functional Assays protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2009 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Acoustic overexposure • Cochlear functional assays • Confocal imaging of inner ear
Primary readouts
- Threshold sensitivity changes
- Cochlear sensory cell integrity
- Afferent nerve terminal loss
- Cochlear nerve degeneration
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Acoustic overexposure
Subjects were exposed to intense sound causing moderate but completely reversible threshold elevation
Note: Exposure caused acute loss of afferent nerve terminals and delayed degeneration of cochlear nerve despite threshold recovery
View evidence from paper
“acoustic overexposures causing moderate, but completely reversible, threshold elevation”
Cochlear functional assays
Assessment of cochlear function to measure threshold sensitivity changes
Note: Conventional threshold testing was performed to assess hearing recovery
View evidence from paper
“using cochlear functional assays and confocal imaging of the inner ear in mouse”
Confocal imaging of inner ear
Microscopic examination of cochlear structures including sensory cells, afferent nerve terminals, and cochlear nerve
Note: Imaging revealed intact cochlear sensory cells but acute loss of afferent nerve terminals and delayed nerve degeneration
View evidence from paper
“confocal imaging of the inner ear in mouse, that acoustic overexposures causing moderate, but completely reversible, threshold elevation leave cochlear sensory cells intact, but cause acute loss of afferent nerve terminals and delayed degeneration of the cochlear nerve”