Cognitive Impairment Testing
Objective: Assess cognitive function 30 days postinjury to measure TBI-associated cognitive impairment
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
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Materials1
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Software1
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Protocol Steps
Microglial Depletion
Administer PLX5622, a CSF1R antagonist, to deplete microglia before injury induction
Note: Treatment administered prior to fluid percussion injury
View evidence from paper
“Microglia were depleted with PLX5622, a CSF1R antagonist, before midline fluid percussion injury (FPI)”
Traumatic Brain Injury Induction
Induce midline fluid percussion injury in male mice
Note: Performed on male mice after microglial depletion
View evidence from paper
“midline fluid percussion injury (FPI) in male mice”
Cognitive Function Assessment
Assess cognitive function at 30 days postinjury to measure TBI-associated cognitive impairment
Note: Critical timepoint for evaluating chronic cognitive impairment; microglial depletion prevented TBI-induced cognitive changes at this timepoint
View evidence from paper
“Cognitive function was assessed 30 days postinjury to measure TBI-associated cognitive impairment”