Cognitive Impairment Testing
Objective: Assess cognitive function 30 days postinjury to measure TBI-associated cognitive impairment
This is a Cognitive Impairment Testing protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 2021 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not specified • male • Not specified • Not specified
Study window
~4.3 week study window
Core workflow
Microglial Depletion • Traumatic Brain Injury Induction • Cognitive Function Assessment
Primary readouts
- Cognitive impairment at 30 days postinjury
- TBI-associated functional and behavioral impairments
- Prevention of cognitive changes by microglial depletion
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Microglial Depletion
Administer PLX5622, a CSF1R antagonist, to deplete microglia before injury induction
Note: Treatment administered prior to fluid percussion injury
View evidence from paper
“Microglia were depleted with PLX5622, a CSF1R antagonist, before midline fluid percussion injury (FPI)”
Traumatic Brain Injury Induction
Induce midline fluid percussion injury in male mice
Note: Performed on male mice after microglial depletion
View evidence from paper
“midline fluid percussion injury (FPI) in male mice”
Cognitive Function Assessment
Assess cognitive function at 30 days postinjury to measure TBI-associated cognitive impairment
Note: Critical timepoint for evaluating chronic cognitive impairment; microglial depletion prevented TBI-induced cognitive changes at this timepoint
View evidence from paper
“Cognitive function was assessed 30 days postinjury to measure TBI-associated cognitive impairment”