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Conditioned Place Aversion — Benjamin B. Land | ReplicateScience

Experiments/Conditioned Place Aversion

Source Paper

The Dysphoric Component of Stress Is Encoded by Activation of the Dynorphin κ-Opioid System

Benjamin B. Land, Michael R. Bruchas, Julia C. Lemos, Mei Xu, Erica J. Melief et al.

Journal of Neuroscience • 2008

Conditioned Place Aversion

behavioralmouseNot specified in provided text

Objective: Measurement of place aversion induced by CRF, urocortin III, or KOR agonist injection to assess dysphoric component of stress encoded by dynorphin κ-opioid system

Materials & Equipment Checklist

9 items1 from ConductScience

Gather these items before starting the experiment. Check off items as you prepare.

Equipment1

Conditioned place aversion apparatus

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Materials7

Corticotropin-releasing factor (CRF)

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Urocortin III

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U50,488

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Antisauvigine-30

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Antalarmin

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κ-opioid receptor (KOR) antagonist

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Phospho-selective antibody directed against activated KOR

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Software1

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1 item available from ConductScience

Estimated: $1,290.00

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Protocol Steps

1

Drug injection and place aversion testing

Mice received injections of CRF, urocortin III, or KOR agonist U50,488 and were tested for place aversion behavior

Not specified in provided textNot specified in provided text

Conditioned Place Preference - Mouse (Custom Order)

Conditioned Place Preference - Mouse (Custom Order)

Matches: Conditioned place aversion apparatus

$1,290View

Note: Place aversion was measured as the primary outcome

View evidence from paper

“Injection of corticotropin-releasing factor (CRF) or urocortin III, key mediators of the stress response, produced place aversion”

2

Antagonist administration

KOR antagonist, CRF2 receptor antagonist (antisauvigine-30), or CRF1 receptor antagonist (antalarmin) were administered to block place aversion

Not specified in provided textNot specified in provided text

Conditioned Place Preference - Mouse (Custom Order)

Conditioned Place Preference - Mouse (Custom Order)

Matches: Conditioned place aversion apparatus

$1,290View

Note: Different antagonists were tested to determine receptor specificity

View evidence from paper

“CRF-induced place aversion was blocked by the CRF 2 receptor antagonist antisauvigine-30, but not by the CRF 1 receptor antagonist antalarmin”

3

Genetic manipulation comparison

Place aversion responses were compared between wild-type mice and mice lacking dynorphin gene

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Conditioned Place Preference - Mouse (Custom Order)

Conditioned Place Preference - Mouse (Custom Order)

Matches: Conditioned place aversion apparatus

$1,290View

Note: Dynorphin knockout mice showed absent aversive behaviors

View evidence from paper

“aversive behaviors that were blocked by a κ-opioid receptor (KOR) antagonist and absent in mice lacking dynorphin”

4

Brain imaging of KOR activation

Phospho-selective antibody directed against activated KOR was used to image sites of dynorphin action in brain regions

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Note: Imaging revealed KOR activation in basolateral amygdala, nucleus accumbens, dorsal raphe, and hippocampus

View evidence from paper

“stress and CRF each caused dynorphin-dependent KOR activation in the basolateral amygdala, nucleus accumbens, dorsal raphe, and hippocampus”