Source Paper
Mice Lacking the ERK1 Isoform of MAP Kinase Are Unimpaired in Emotional Learning
Joel C. Selcher, Tanya Nekrasova, Richard Paylor, Gary E. Landreth, J. David Sweatt
Learning & Memory • 2001
Contextual Fear Conditioning
Objective: Measurement of acquisition and retention of fear memory to context at 24 hours and 2 weeks post-training in ERK1-null and wild-type mice
This is a Contextual Fear Conditioning protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2001 paper published in Learning & Memory.
Model and subjects
mouse • Not explicitly stated • unknown • Not explicitly stated • Not explicitly stated
Study window
~2 week study window | ~24 hours hands-on
Core workflow
Contextual Fear Conditioning Training • 24-Hour Retention Test • 2-Week Retention Test
Primary readouts
- Acquisition of contextual fear conditioning
- Retention of contextual fear memory at 24 hours post-training
- Retention of contextual fear memory at 2 weeks post-training
- Comparison between ERK1-null and wild-type littermate controls
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Contextual Fear Conditioning Training
Mice undergo fear conditioning training to establish baseline fear memory to context
Note: Training phase for acquisition of contextual fear memory
View evidence from paper
“acquisition or retention (24 h and 2 wk after training) of either contextual or cue fear conditioning”
24-Hour Retention Test
Assessment of contextual fear memory retention 24 hours after training
Note: First retention measurement timepoint
View evidence from paper
“retention (24 h and 2 wk after training) of either contextual or cue fear conditioning”
2-Week Retention Test
Assessment of contextual fear memory retention 2 weeks after training
Note: Second retention measurement timepoint
View evidence from paper
“retention (24 h and 2 wk after training) of either contextual or cue fear conditioning”