Contextual Fear Conditioning
Objective: Assessment of fear memory and cognitive function in response to contextual cues in CX3CR1-deficient mice
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Protocol Steps
Contextual Fear Conditioning Test
Mice were subjected to contextual fear conditioning to assess fear memory and cognitive function in response to contextual cues
Note: Test was performed on CX3CR1 -/-, CX3CR1 +/-, and wild-type mice to compare cognitive deficits
View evidence from paper
“mice lacking the CX3CR1 receptor show contextual fear conditioning and Morris water maze deficits”
Morris Water Maze Testing
Spatial learning and memory assessment was conducted using the Morris water maze
Note: Performed alongside contextual fear conditioning to evaluate cognitive function
View evidence from paper
“mice lacking the CX3CR1 receptor show contextual fear conditioning and Morris water maze deficits”
Motor Learning Assessment
Motor learning was evaluated in the different genotype groups
Note: CX3CR1 deficiency was found to affect motor learning
View evidence from paper
“CX3CR1 deficiency also affects motor learning”
IL-1β Receptor Antagonist Infusion
IL-1β receptor antagonist was infused to test whether it could reverse cognitive deficits and LTP impairment
Note: Infusion significantly reversed the deficit in cognitive function and impairment in LTP
View evidence from paper
“Infusion with IL-1β receptor antagonist significantly reversed the deficit in cognitive function and impairment in LTP”