Source Paper
Hyperactivity and Intact Hippocampus-Dependent Learning in Mice Lacking the M<sub>1</sub>Muscarinic Acetylcholine Receptor
Tsuyoshi Miyakawa, Masahisa Yamada, Alokesh Duttaroy, Jürgen Wess
Journal of Neuroscience • 2001
Contextual Fear Conditioning
Objective: Assessment of hippocampus-dependent associative learning through context-shock pairing in M1 receptor-deficient mice
This is a Contextual Fear Conditioning protocol using mouse as the model organism. The procedure involves 1 procedural steps, 1 equipment items. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
mouse • M1R−/− mice (M1 receptor-deficient) and wild-type controls • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Contextual Fear Conditioning Test
Primary readouts
- Freezing behavior after inescapable footshocks
- Cognitive performance in contextual fear conditioning
- Association between context and shock
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Contextual Fear Conditioning Test
Mice were subjected to contextual fear conditioning to assess hippocampus-dependent associative learning through context-shock pairing
Note: This test measures the ability to associate environmental context with an aversive stimulus (footshock)
View evidence from paper
“M1R−/− mice displayed no significant cognitive impairments in the Morris water maze and in contextual fear conditioning”