Controlled Cortical Impact
Objective: To examine the efficacy of fluoxetine after moderate to severe traumatic brain injury produced by controlled cortical impact, evaluating effects on hippocampal neurogenesis, epigenetic modifications, and functional outcomes including gait and memory
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Materials2
Software1
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Protocol Steps
Traumatic brain injury induction
Moderate to severe traumatic brain injury was induced in mice using controlled cortical impact
Note: Sham surgery was performed on control animals
View evidence from paper
“a moderate to severe TBI, produced by a controlled cortical impact”
Treatment initiation
Three days after TBI or sham surgery, mice were treated with fluoxetine or vehicle
Note: Treatment began 3 days post-injury
View evidence from paper
“Three days after TBI or sham surgery, mice were treated with fluoxetine (10 mg/kg/d) or vehicle for 4 weeks”
Hippocampal neurogenesis assessment
Stereologic analysis of the dentate gyrus was performed to evaluate doublecortin-positive cells as a marker of neurogenesis
Note: Doublecortin-positive cells were quantified in brain-injured animals
View evidence from paper
“Stereologic analysis of the dentate gyrus revealed a significant increase in doublecortin-positive cells in brain-injured animals treated with fluoxetine”
Epigenetic modification analysis
Immunohistochemistry and quantitative Western immunoblots were performed to assess histone 3 acetylation and methyl-CpG-binding protein expression
Note: Epigenetic modifications included histone 3 acetylation and induction of methyl-CpG-binding protein
View evidence from paper
“Epigenetic modifications, including an increase in histone 3 acetylation and induction of methyl-CpG-binding protein, were likewise seen by immunohistochemistry and quantitative Western immunoblots”
Gait function assessment
Gait function was assessed using the CatWalk-assisted gait test
Note: Compared between fluoxetine- and vehicle-treated animals
View evidence from paper
“gait function and spatial learning and memory were assessed by the CatWalk-assisted gait test and Barnes maze test”
Spatial learning and memory assessment
Spatial learning and memory were assessed using the Barnes maze test
Note: Compared between fluoxetine- and vehicle-treated animals
View evidence from paper
“gait function and spatial learning and memory were assessed by the CatWalk-assisted gait test and Barnes maze test”