Corner Test
Objective: Evaluation of sensorimotor asymmetry by measuring preferential use of one side when exiting a corner
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Induce transient or permanent middle cerebral artery occlusion
Perform either transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO) in wild-type (WT) and IL-4 knockout (KO) C57/BL6 mice
Note: Two different occlusion methods used in different animal groups
View evidence from paper
“Transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO) was induced in wild-type (WT) and IL-4 knockout (KO) C57/BL6 mice”
Administer IL-4 or vehicle infusion
In a separate cohort of WT animals, infuse IL-4 (60 ng/d for 7d) or vehicle into the cerebroventricle after tMCAO
Note: This step applies only to the separate WT cohort, not to the KO animals
View evidence from paper
“In a separate cohort of WT animals, IL-4 (60 ng/d for 7d) or vehicle was infused into the cerebroventricle after tMCAO”
Perform corner test
Assess sensorimotor asymmetry by measuring preferential use of one side when exiting a corner as part of behavioral outcome assessment
Note: Corner test is one of multiple behavioral tests performed
View evidence from paper
“Behavioral outcomes were assessed by the Rotarod, corner, foot fault, and Morris water maze tests”