Corner Test
Objective: Evaluation of sensorimotor asymmetry by measuring preferential use of one side when exiting a corner
This is a Corner Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2016 paper published in Stroke.
Model and subjects
mouse • C57/BL6 • unknown • Not specified • Not specified
Study window
~1 week study window
Core workflow
Induce transient or permanent middle cerebral artery occlusion • Administer IL-4 or vehicle infusion • Perform corner test
Primary readouts
- Preferential use of one side when exiting a corner (sensorimotor asymmetry)
- Neuronal tissue loss verified by two independent neuron markers
- Markers of classically activated (M1) and alternatively activated (M2) microglia
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Induce transient or permanent middle cerebral artery occlusion
Perform either transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO) in wild-type (WT) and IL-4 knockout (KO) C57/BL6 mice
Note: Two different occlusion methods used in different animal groups
View evidence from paper
“Transient middle cerebral artery occlusion (tMCAO) or permanent distal MCAO (dMCAO) was induced in wild-type (WT) and IL-4 knockout (KO) C57/BL6 mice”
Administer IL-4 or vehicle infusion
In a separate cohort of WT animals, infuse IL-4 (60 ng/d for 7d) or vehicle into the cerebroventricle after tMCAO
Note: This step applies only to the separate WT cohort, not to the KO animals
View evidence from paper
“In a separate cohort of WT animals, IL-4 (60 ng/d for 7d) or vehicle was infused into the cerebroventricle after tMCAO”
Perform corner test
Assess sensorimotor asymmetry by measuring preferential use of one side when exiting a corner as part of behavioral outcome assessment
Note: Corner test is one of multiple behavioral tests performed
View evidence from paper
“Behavioral outcomes were assessed by the Rotarod, corner, foot fault, and Morris water maze tests”