Source Paper
Propranolol Administration During Morphine Addiction Attenuates Reinstatement of Drug-Aversive Memories Caused by Exposure to Stressful Stimuli
Cánovas-Cabanes A, Teruel-Fernández FJ, Fernández-López L, Martínez-Laorden E, Navarro-Zaragoza J et al.
Pharmaceuticals (Basel) • 2025
CPA Extinction
Objective: To extinguish conditioned place aversion (CPA) associated with naloxone-induced opioid withdrawal before testing reinstatement
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Protocol Steps
Timing of extinction initiation
Begin extinction sessions 24 hours after the CPA post-test
View evidence from paper
“Extinction sessions began 24 h after the CPA post-test”
Duration and frequency
Conduct daily extinction sessions for 20-25 days until extinction criteria are met
View evidence from paper
“were conducted daily for 20–25 days until extinction criteria were met”
Session procedure
Place mice in central passageway and allow free access to all chambers for 15 minutes
View evidence from paper
“mice were placed in the central passageway and allowed free access to all chambers for 15 min”
Data collection
Record time spent in naloxone-paired chamber and number of crossings between compartments
View evidence from paper
“Time spent in the naloxone-paired chamber and the number of crossings between compartments were recorded”
Extinction criteria confirmation
Confirm extinction using paired t-test analyses comparing post-test and final extinction values
View evidence from paper
“Extinction was confirmed using paired t-test analyses comparing post-test and final extinction values”