Source Paper
Propranolol Administration During Morphine Addiction Attenuates Reinstatement of Drug-Aversive Memories Caused by Exposure to Stressful Stimuli
Cánovas-Cabanes A, Teruel-Fernández FJ, Fernández-López L, Martínez-Laorden E, Navarro-Zaragoza J et al.
Pharmaceuticals (Basel) • 2025
CPA Extinction
Objective: To extinguish conditioned place aversion (CPA) associated with naloxone-induced opioid withdrawal before testing reinstatement
This is a CPA Extinction protocol using Mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Pharmaceuticals (Basel).
Model and subjects
Mouse • C57BL/6J • Male • Adult • 179
Study window
~3.6 week study window | ~15 minutes hands-on
Core workflow
Timing of extinction initiation • Duration and frequency • Session procedure
Primary readouts
- Time spent in naloxone-paired chamber
- Number of crossings between compartments
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Timing of extinction initiation
Begin extinction sessions 24 hours after the CPA post-test
View evidence from paper
“Extinction sessions began 24 h after the CPA post-test”
Duration and frequency
Conduct daily extinction sessions for 20-25 days until extinction criteria are met
View evidence from paper
“were conducted daily for 20–25 days until extinction criteria were met”
Session procedure
Place mice in central passageway and allow free access to all chambers for 15 minutes
View evidence from paper
“mice were placed in the central passageway and allowed free access to all chambers for 15 min”
Data collection
Record time spent in naloxone-paired chamber and number of crossings between compartments
View evidence from paper
“Time spent in the naloxone-paired chamber and the number of crossings between compartments were recorded”
Extinction criteria confirmation
Confirm extinction using paired t-test analyses comparing post-test and final extinction values
View evidence from paper
“Extinction was confirmed using paired t-test analyses comparing post-test and final extinction values”