Propranolol Administration During Morphine Addiction Attenuates Reinstatement of Drug-Aversive Memories Caused by Exposure to Stressful Stimuli

Cánovas-Cabanes A, Teruel-Fernández FJ, Fernández-López L, Martínez-Laorden E, Navarro-Zaragoza J et al.

Pharmaceuticals (Basel) • 2025

CPA Reinstatement

behavioralMouseC57BL/6J

Objective: To assess the re-emergence of previously extinguished conditioned place aversion following exposure to stressors

Materials & Equipment Checklist

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Ensure extinction criteria are met through daily 20-25 day sessions until paired t-test confirms extinction

View evidence from paper

“Extinction sessions began 24 h after the CPA post-test and were conducted daily for 20–25 days until extinction criteria were met”

Inject propranolol (10 mg/kg, i.p.) or vehicle one day after extinction, timing varies by stressor type

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“mice were randomly assigned to receive either the β-ARs antagonist propranolol at a dose of 10 mg/kg (i.p.) or its vehicle (saline)”

Expose mice to social defeat (15-min agonistic encounter), restraint stress (15 min in conical tubes), or tail-pinch (15 min with paper clip)

View evidence from paper

“exposing mice to either an acute social defeat or a physical stressor (restraint or tail-pinch)”

Place mice in central passageway and allow free access to all chambers for 15 minutes in a different room from conditioning

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“allowing animals free access to all compartments of the apparatus for 15 min”

Measure time spent in naloxone-paired chamber and number of crossings between compartments

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“Time spent in the naloxone-paired chamber and the number of crossings between compartments were recorded”