Source Paper
Propranolol Administration During Morphine Addiction Attenuates Reinstatement of Drug-Aversive Memories Caused by Exposure to Stressful Stimuli
Cánovas-Cabanes A, Teruel-Fernández FJ, Fernández-López L, Martínez-Laorden E, Navarro-Zaragoza J et al.
Pharmaceuticals (Basel) • 2025
CPA Reinstatement
Objective: To assess the re-emergence of previously extinguished conditioned place aversion following exposure to stressors
This is a CPA Reinstatement protocol using Mouse as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 5 materials. Extracted from a 2025 paper published in Pharmaceuticals (Basel).
Model and subjects
Mouse • C57BL/6J • Male • Adult male • 179
Study window
~3.6 week study window | ~45 minutes hands-on
Core workflow
Complete CPA extinction • Administer propranolol (if applicable) • Apply stressor
Primary readouts
- Time spent in naloxone-paired chamber
- Number of crossings between compartments
- Re-emergence of conditioned place aversion
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Complete CPA extinction
Ensure extinction criteria are met through daily 20-25 day sessions until paired t-test confirms extinction
View evidence from paper
“Extinction sessions began 24 h after the CPA post-test and were conducted daily for 20–25 days until extinction criteria were met”
Administer propranolol (if applicable)
Inject propranolol (10 mg/kg, i.p.) or vehicle one day after extinction, timing varies by stressor type
View evidence from paper
“mice were randomly assigned to receive either the β-ARs antagonist propranolol at a dose of 10 mg/kg (i.p.) or its vehicle (saline)”
Apply stressor
Expose mice to social defeat (15-min agonistic encounter), restraint stress (15 min in conical tubes), or tail-pinch (15 min with paper clip)
View evidence from paper
“exposing mice to either an acute social defeat or a physical stressor (restraint or tail-pinch)”
Conduct reinstatement test
Place mice in central passageway and allow free access to all chambers for 15 minutes in a different room from conditioning
View evidence from paper
“allowing animals free access to all compartments of the apparatus for 15 min”
Record behavioral measures
Measure time spent in naloxone-paired chamber and number of crossings between compartments
View evidence from paper
“Time spent in the naloxone-paired chamber and the number of crossings between compartments were recorded”