Source Paper
Mice Lacking the ERK1 Isoform of MAP Kinase Are Unimpaired in Emotional Learning
Joel C. Selcher, Tanya Nekrasova, Richard Paylor, Gary E. Landreth, J. David Sweatt
Learning & Memory • 2001
Cue Fear Conditioning
Objective: Measurement of acquisition and retention of fear memory to an auditory cue at 24 hours and 2 weeks post-training in mice
This is a Cue Fear Conditioning protocol using mouse as the model organism. The procedure involves 3 procedural steps. Extracted from a 2001 paper published in Learning & Memory.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
~2 week study window | ~24 hours hands-on
Core workflow
Cue Fear Conditioning Training • 24-Hour Retention Test • 2-Week Retention Test
Primary readouts
- Acquisition of fear memory to auditory cue
- Retention of fear memory at 24 hours post-training
- Retention of fear memory at 2 weeks post-training
- Comparison between ERK1-null and wild-type littermate controls
Key equipment and reagents
Verified items
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Protocol Steps
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Cue Fear Conditioning Training
Mice underwent cue fear conditioning training to establish fear memory to an auditory cue
Note: Training involved pairing an auditory cue with an unconditioned stimulus
View evidence from paper
“no differences were observed in the acquisition or retention (24 h and 2 wk after training) of either contextual or cue fear conditioning”
24-Hour Retention Test
Assessment of fear memory retention to the auditory cue at 24 hours post-training
Note: Retention was measured in both ERK1-null and wild-type littermate controls
View evidence from paper
“no differences were observed in the acquisition or retention (24 h and 2 wk after training) of either contextual or cue fear conditioning”
2-Week Retention Test
Assessment of fear memory retention to the auditory cue at 2 weeks post-training
Note: Long-term retention was measured in both ERK1-null and wild-type littermate controls
View evidence from paper
“no differences were observed in the acquisition or retention (24 h and 2 wk after training) of either contextual or cue fear conditioning”