Source Paper
Prepulse Inhibition Deficits and Perseverative Motor Patterns in Dopamine Transporter Knock-Out Mice: Differential Effects of D1 and D2 Receptor Antagonists
Rebecca J. Ralph, Martin P. Paulus, Fabio Fumagalli, Marc G. Caron, Mark A. Geyer
Journal of Neuroscience • 2001
D1 Receptor Antagonist Challenge with SCH23390
Objective: Examine effects of D1 receptor antagonist SCH23390 on prepulse inhibition and perseverative motor patterns in DAT knock-out mice
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Protocol Steps
Baseline behavioral assessment
Establish baseline measurements of prepulse inhibition, motor patterns, and activity levels in DAT knock-out mice and wild-type controls
Note: DAT (-/-) mice exhibited deficient sensorimotor gating, nonfocal perseverative locomotion patterns, and hyperactivity
View evidence from paper
“At baseline, DAT (−/−) mice exhibited deficient sensorimotor gating as measured by prepulse inhibition (PPI) of the startle response, exhibited nonfocal perseverative patterns of locomotion, and were hyperactive in a novel environment”
SCH23390 pretreatment
Administer D1 receptor antagonist SCH23390 as pretreatment prior to behavioral testing
Note: Dose and route of administration not specified in provided text
View evidence from paper
“Pretreatment with raclopride significantly increased PPI in the DAT (−/−) mice, whereas SCH23390 had no significant effect”
Prepulse inhibition testing
Measure prepulse inhibition of the startle response following SCH23390 pretreatment
Note: SCH23390 had no significant effect on PPI in DAT knock-out mice
View evidence from paper
“SCH23390 had no significant effect”
Motor behavior pattern analysis
Assess perseverative motor patterns and locomotor behavior following D1 receptor antagonism
Note: D1 receptor antagonism significantly attenuated perseverative patterns, producing more meandering behavior
View evidence from paper
“antagonism of D1 receptors significantly attenuated the perseverative patterns, producing more of a meandering behavior seen in the DAT (+/+) control mice”
Hyperactivity measurement
Quantify total motor activity and hyperactivity levels following SCH23390 administration
Note: D1 receptor antagonist decreased hyperactivity in DAT knock-out mice
View evidence from paper
“Both D1 and D2 receptor antagonists decreased the hyperactivity seen in the DAT (−/−) mice”