Source Paper
Prepulse Inhibition Deficits and Perseverative Motor Patterns in Dopamine Transporter Knock-Out Mice: Differential Effects of D1 and D2 Receptor Antagonists
Rebecca J. Ralph, Martin P. Paulus, Fabio Fumagalli, Marc G. Caron, Mark A. Geyer
Journal of Neuroscience • 2001
D2 Receptor Antagonist Challenge with Raclopride
Objective: To examine the effects of D2 receptor antagonist raclopride on prepulse inhibition and motor behavior in dopamine transporter knock-out mice
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Materials2
Software1
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Protocol Steps
Baseline behavioral assessment
Establish baseline measurements of prepulse inhibition, motor behavior patterns, and activity levels in DAT (−/−) mice and DAT (+/+) control mice
Note: DAT (−/−) mice exhibited deficient sensorimotor gating, nonfocal perseverative locomotion patterns, and hyperactivity
View evidence from paper
“At baseline, DAT (−/−) mice exhibited deficient sensorimotor gating as measured by prepulse inhibition (PPI) of the startle response, exhibited nonfocal perseverative patterns of locomotion, and were hyperactive in a novel environment”
Raclopride pretreatment
Administer raclopride (D2 receptor antagonist) as pretreatment to DAT (−/−) mice
Note: Dose and route of administration not specified
View evidence from paper
“Pretreatment with raclopride significantly increased PPI in the DAT (−/−) mice”
SCH23390 pretreatment
Administer SCH23390 (D1 receptor antagonist) as pretreatment to DAT (−/−) mice for comparison
Note: Dose and route of administration not specified
View evidence from paper
“SCH23390 had no significant effect”
Measure prepulse inhibition
Measure prepulse inhibition of the startle response following drug pretreatment
Note: Raclopride significantly increased PPI in DAT (−/−) mice; SCH23390 had no significant effect
View evidence from paper
“Pretreatment with raclopride significantly increased PPI in the DAT (−/−) mice, whereas SCH23390 had no significant effect”
Assess motor behavior patterns
Evaluate motor behavior patterns including perseverative locomotion and meandering behavior following drug pretreatment
Note: D2 blockade did not affect straight motor patterns; D1 antagonism attenuated perseverative patterns
View evidence from paper
“Blockade of D2 receptors did not affect the predominantly straight patterns of motor behavior produced by the DAT (−/−) mice, but antagonism of D1 receptors significantly attenuated the perseverative patterns”
Measure motor activity levels
Quantify total motor activity and hyperactivity following drug pretreatment
Note: Both D1 and D2 receptor antagonists decreased hyperactivity
View evidence from paper
“Both D1 and D2 receptor antagonists decreased the hyperactivity seen in the DAT (−/−) mice”