Source Paper
Systemic Interplay of BDNF and Serotonin Pathways Defines Behavioral and Molecular Responses to Midbrain 5-HT7 Overexpression and Chronic Ethanol Consumption
Rodnyy A, Oreshko A, Eremin D, Naumenko V, Bazovkina D
Biomolecules • 2026
Dark-Light Box Test
Objective: To measure anxiety-like behavior by assessing time spent in light vs dark compartments and exploratory behavior
This is a Dark-Light Box Test protocol using Mouse as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in Biomolecules.
Model and subjects
Mouse • C57BL/6 • Male • 8–10 weeks • 40
Study window
~5 minutes hands-on
Core workflow
Animal placement • Behavioral recording
Primary readouts
- Duration of time spent in the light compartment
- Proportion of the light area explored
- Number of entries into the light compartment from the dark
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Animal placement
Place mice in the light compartment facing the opening between compartments
View evidence from paper
“Mice were placed in the light compartment, facing the opening”
Behavioral recording
Record mouse behavior for 5 minutes, measuring time in light compartment, proportion of light area explored, and number of entries into light compartment
View evidence from paper
“Over a 5 min session, the following parameters were recorded: the duration of time spent in the light compartment, the proportion of the light area explored, and the number of entries into the light compartment from the dark”