Source Paper
Systemic Interplay of BDNF and Serotonin Pathways Defines Behavioral and Molecular Responses to Midbrain 5-HT7 Overexpression and Chronic Ethanol Consumption
Rodnyy A, Oreshko A, Eremin D, Naumenko V, Bazovkina D
Biomolecules • 2026
Dark-Light Box Test
behavioralMouseC57BL/6
Objective: To measure anxiety-like behavior by assessing time spent in light vs dark compartments and exploratory behavior
Protocol Steps
1
Animal placement
Place mice in the light compartment facing the opening between compartments
View evidence from paper
“Mice were placed in the light compartment, facing the opening”
2
Behavioral recording
Record mouse behavior for 5 minutes, measuring time in light compartment, proportion of light area explored, and number of entries into light compartment
View evidence from paper
“Over a 5 min session, the following parameters were recorded: the duration of time spent in the light compartment, the proportion of the light area explored, and the number of entries into the light compartment from the dark”