DAT Knockout Mouse Sleep Analysis
Objective: Assessment of sleep-wake cycle parameters and wakefulness consolidation in dopamine transporter gene-deleted mice, and evaluation of responsiveness to wake-promoting compounds
This is a DAT Knockout Mouse Sleep Analysis protocol using mouse as the model organism. The procedure involves 8 procedural steps, 2 equipment items, 5 materials. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
mouse • DAT knockout mice (dopamine transporter gene-deleted) • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Polygraphic Recording Setup • Baseline Sleep-Wake Measurement • Modafinil Administration and Response Testing
Primary readouts
- Non-rapid eye movement (NREM) sleep time
- Wakefulness consolidation
- Wake-promoting response to modafinil
- Wake-promoting response to amphetamine
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Polygraphic Recording Setup
Establish polygraphic recordings to measure sleep-wake cycle parameters in DAT knockout mice and control animals
Note: Recordings used to assess non-rapid eye movement sleep time and wakefulness consolidation
View evidence from paper
“polygraphic recordings and caudate microdialysate dopamine measurements in narcoleptic dogs revealed”
Baseline Sleep-Wake Measurement
Record baseline sleep-wake cycle parameters in DAT knockout mice prior to drug administration
Note: Establish baseline non-rapid eye movement sleep time and wakefulness consolidation measures
View evidence from paper
“deletion of the dopamine transporter (DAT) gene reduced non-rapid eye movement sleep time and increased wakefulness consolidation”
Modafinil Administration and Response Testing
Administer modafinil to DAT knockout mice and measure wake-promoting response
Note: DAT knockout mice showed no response to modafinil despite normal responsiveness in wild-type controls
View evidence from paper
“DAT knock-out mice were also unresponsive to the normally robust wake-promoting action of modafinil”
Methamphetamine Administration and Response Testing
Administer methamphetamine to DAT knockout mice and measure wake-promoting response
Note: DAT knockout mice showed no response to methamphetamine
View evidence from paper
“DAT knock-out mice were also unresponsive to the normally robust wake-promoting action of methamphetamine”
GBR12909 Administration and Response Testing
Administer selective DAT blocker GBR12909 to DAT knockout mice and measure wake-promoting response
Note: DAT knockout mice showed no response to GBR12909
View evidence from paper
“selective DAT blocker GBR12909 but were hypersensitive to the wake-promoting effects of caffeine”
Caffeine Administration and Response Testing
Administer caffeine to DAT knockout mice and measure wake-promoting response
Note: DAT knockout mice showed hypersensitivity to caffeine wake-promoting effects
View evidence from paper
“were hypersensitive to the wake-promoting effects of caffeine”
Dopamine Measurement
Measure extracellular dopamine levels in caudate region during drug administration
Note: Microdialysis measurements to assess dopamine changes in response to wake-promoting compounds
View evidence from paper
“caudate microdialysate dopamine measurements in narcoleptic dogs revealed that the wake-promoting antinarcoleptic compounds modafinil and amphetamine increase extracellular dopamine”
Locomotor Activity Assessment
Measure locomotor activity to distinguish sleep-wake effects from general motor effects
Note: Wakefulness consolidation effects were independent from locomotor effects
View evidence from paper
“deletion of the dopamine transporter (DAT) gene reduced non-rapid eye movement sleep time and increased wakefulness consolidation independently from locomotor effects”