Depression and Anxiety-like Behavioral Paradigms
Objective: Evaluation of behavioral sensitivity to antidepressive treatments using depression and anxiety-like behavior assays to determine the role of TrkB in hippocampal neurogenesis and antidepressant response
Protocol Steps
Behavioral Testing in Depression and Anxiety-like Paradigms
Mice were exposed to depression and anxiety-like behavioral paradigms to assess behavioral sensitivity to antidepressive treatment
Note: Testing was conducted on both TrkB-deficient mice in hippocampal neural progenitor cells and control mice with TrkB only lacking in differentiated dentate gyrus neurons
View evidence from paper
“Ablation of TrkB also renders these mice behaviorally insensitive to antidepressive treatment in depression and anxiety-like paradigms”
Chronic Antidepressant Administration
Mice received chronic antidepressant treatment prior to behavioral testing
Note: Antidepressant type and dosage not specified in provided text
View evidence from paper
“Adult hippocampal neurogenesis is stimulated by chronic administration of antidepressants”
Behavioral Assessment and Comparison
Behavioral responses to antidepressive treatment were compared between TrkB-deficient mice and control mice with normal TrkB expression in differentiated neurons
Note: TrkB-deficient mice showed no behavioral sensitivity to antidepressive treatment, while control mice responded normally
View evidence from paper
“In contrast, mice lacking TrkB only in differentiated DG neurons display typical neurogenesis and respond normally to chronic AD”