Depression-Like Behavior Testing
Objective: Measurement of depression-like and anxiety-related behaviors in mice under baseline conditions and chronic stress conditions using corticosterone administration
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Materials1
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Protocol Steps
Baseline anxiety and depression-related behavior assessment
Measure anxiety and depression-related behaviors in mice under baseline conditions without stress treatment
Note: No effects on baseline anxiety and depression-related behavior were found
View evidence from paper
“We find no effects on baseline anxiety and depression-related behavior”
Chronic corticosterone administration
Treat mice chronically with corticosterone to induce a stress model
Note: This creates a chronic stress condition in the mouse model
View evidence from paper
“mice treated chronically with corticosterone (CORT), a mouse model of stress”
Anxiety and depression-related behavior assessment under chronic stress
Measure anxiety and depression-related behaviors in mice following chronic corticosterone treatment
Note: Increased adult neurogenesis was found to reduce anxiety and depression-related behaviors under chronic stress conditions
View evidence from paper
“we find that increasing adult neurogenesis is sufficient to reduce anxiety and depression-related behaviors in mice treated chronically with corticosterone”
HPA axis regulation assessment
Evaluate hypothalamic-pituitary-adrenal axis regulation at baseline and following chronic corticosterone administration
Note: No effect of increased adult hippocampal neurogenesis on HPA axis regulation was found
View evidence from paper
“we find no effect of increased adult hippocampal neurogenesis on hypothalamic–pituitary–adrenal (HPA) axis regulation, either at baseline or following chronic CORT administration”