Developmental Delay Assessment
Objective: Evaluation of global developmental delays in Adnp+/- mice with and without NAP treatment to assess developmental and behavioral phenotypes
This is a Developmental Delay Assessment protocol using mouse as the model organism. The procedure involves 8 procedural steps, 1 materials. Extracted from a 2018 paper published in Journal of Clinical Investigation.
Model and subjects
mouse • Adnp+/- (haploinsufficient mutant line) • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Establish experimental groups • Assess global developmental delays • Measure vocalization impediments
Primary readouts
- Global developmental delays
- Vocalization impediments
- Gait and motor dysfunctions
- Social memory impairments
Key equipment and reagents
Verified items
0
Direct vendor links
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Protocol Steps
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Establish experimental groups
Create two groups of Adnp+/- mice: one receiving NAP treatment and one control group without treatment
Note: Treatment administered daily via systemic or nasal routes
View evidence from paper
“Adnp+/- mice further exhibited global developmental delays, vocalization impediments, gait and motor dysfunctions, and social and object memory impairments, all of which were partially reversed by daily NAP administration (systemic/nasal)”
Assess global developmental delays
Evaluate developmental milestones and delays in Adnp+/- mice with and without NAP treatment
Note: Specific assessment methods not detailed in provided text
View evidence from paper
“Adnp+/- mice further exhibited global developmental delays, vocalization impediments, gait and motor dysfunctions”
Measure vocalization impediments
Assess vocalization deficits in treated and untreated Adnp+/- mice
Note: Specific measurement methodology not provided in text
View evidence from paper
“vocalization impediments, gait and motor dysfunctions, and social and object memory impairments”
Evaluate gait and motor function
Assess motor dysfunctions and gait abnormalities in both treatment groups
Note: Specific testing apparatus not mentioned in provided text
View evidence from paper
“gait and motor dysfunctions, and social and object memory impairments, all of which were partially reversed by daily NAP administration”
Test social and object memory
Assess social memory and object recognition memory in treated and untreated mice
Note: Specific behavioral testing paradigms not detailed in provided text
View evidence from paper
“social and object memory impairments, all of which were partially reversed by daily NAP administration”
Quantify synaptic pathology in vivo
Use GFP-expressing neuronal membrane-tagged Adnp+/- mice to quantify dendritic spine density and synaptic changes
Note: Unique transgenic line allows direct visualization of synaptic pathology
View evidence from paper
“established a unique neuronal membrane-tagged, GFP-expressing Adnp+/- mouse line allowing in vivo synaptic pathology quantification”
Measure dendritic spine density
Quantify dendritic spine density changes in response to NAP treatment
Note: Reduced spine density was partly ameliorated by NAP treatment
View evidence from paper
“Adnp deficiency reduced dendritic spine density and altered synaptic gene expression, both of which were partly ameliorated by NAP treatment”
Analyze synaptic gene expression
Assess changes in synaptic gene expression in Adnp+/- mice with and without NAP treatment
Note: Gene expression alterations were partly ameliorated by NAP treatment
View evidence from paper
“altered synaptic gene expression, both of which were partly ameliorated by NAP treatment”