Source Paper
Age-related changes in behavior in C57BL/6J mice from young adulthood to middle age
Hirotaka Shoji, Keizo Takao, Satoko Hattori, Tsuyoshi Miyakawa
Molecular Brain • 2016
Elevated Plus Maze
Objective: Measure anxiety-related behavior through exploration of open and closed arms in the elevated plus maze
This is a Elevated Plus Maze protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2016 paper published in Molecular Brain.
Model and subjects
mouse • C57BL/6J and genetically engineered mice with C57BL/6J background • male • Not specified • Not specified • 1739
Study window
~1 day study window
Core workflow
Animal housing and maintenance • Test scheduling and timing • Apparatus cleaning
Primary readouts
- Exploration of open arms
- Exploration of closed arms
- Anxiety-related behavior
Key equipment and reagents
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Protocol Steps
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Animal housing and maintenance
Mice housed in plastic cages with sterilized PaperClean Bedding under 12-hour light/dark cycle with lights on at 7:00 am. Food and water provided ad libitum.
Note: Housing density varied: 1.3% with 1 animal, 2.3% with 2, 6.7% with 3, 80.1% with 4, 8.9% with 5, and 0.7% with 6 animals per cage at test battery start
View evidence from paper
“housed in plastic cages with sterilized PaperClean Bedding (Japan SLC) under a 12-hr light/dark cycle (lights on at 7:00 am) with access to food (CRF-1, Oriental Yeast Co., Ltd.) and water ad libitum”
Test scheduling and timing
Elevated plus maze test performed as part of behavioral test battery. Behavioral testing conducted between 9:00 a.m. and 6:00 p.m. with at least 1 day interval between tests.
Note: Test order: general health/neurological screening, light/dark transition, open field, elevated plus maze, hot plate, social interaction, rotarod, startle response/prepulse inhibition, Porsolt forced swim, Barnes maze, contextual and cued fear conditioning, tail suspension tests
View evidence from paper
“Behavioral testing was performed between 9:00 a.m. and 6:00 p.m. The interval between tests was at least 1 day.”
Apparatus cleaning
After testing, all apparatus cleaned with super hypochlorous water and 70% ethanol to prevent bias due to olfactory cues.
Note: Cleaning performed between tests to eliminate olfactory cues
View evidence from paper
“After the tests, all apparatus were cleaned with super hypochlorous water and 70% ethanol to prevent a bias due to olfactory cues.”