Source Paper
Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice
Stefano Tarantini, Marta Noa Valcarcel-Ares, Peter Toth, Andriy Yabluchanskiy, Zsuzsanna Tucsek et al.
Redox Biology • 2019
Elevated Plus Maze Learning Protocol
Objective: Assessment of learning capacity and hippocampal function by measuring transfer latency on the elevated plus maze across two days
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Protocol Steps
Animal placement on elevated plus maze
Mice were placed individually at the end of an open arm with their back to the central platform
Note: Individual placement ensures consistent starting position
View evidence from paper
“Mice were placed individually at the end of an open arm with their back to the central platform.”
Measurement of transfer latency on Day 1
The time for mice to cross a line halfway along one of the closed arms was measured. Mice had to have their body and each paw on the other side of the line.
Note: If a mouse had not crossed the line after 120 seconds, it was placed beyond it
View evidence from paper
“The time for mice to cross a line halfway along one of the closed arms was measured (transfer latency) on day 1 and day 2. Mice had to have their body and each paw on the other side of the line. If a mouse had not crossed the line after 120 s, it was placed beyond it.”
Post-crossing exploration period on Day 1
After crossing the line, mice had 30 seconds for exploring the apparatus
Note: Allows mice to explore the closed arm area
View evidence from paper
“After crossing the line, mice had 30 s for exploring the apparatus.”
Measurement of transfer latency on Day 2
The same procedure was repeated on day 2, measuring transfer latency time to cross the line halfway along one of the closed arms
Note: Day 2 measurement allows assessment of learning from Day 1
View evidence from paper
“The time for mice to cross a line halfway along one of the closed arms was measured (transfer latency) on day 1 and day 2.”