Elevated Plus Maze
Objective: Assessment of anxiety-like behavior in response to ghrelin administration or calorie restriction using the elevated plus maze test
This is a Elevated Plus Maze protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 2008 paper published in Nature Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Ghrelin Administration via Subcutaneous Injection • Calorie Restriction Treatment • Elevated Plus Maze Testing
Primary readouts
- Anxiety-like behavior in elevated plus maze
- Antidepressant-like responses
- Effects of ghrelin administration on behavioral outcomes
- Effects of calorie restriction on behavioral outcomes
Key equipment and reagents
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Protocol Steps
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Ghrelin Administration via Subcutaneous Injection
Ghrelin was administered to mice through subcutaneous injections to increase ghrelin levels
Note: This is one method of increasing ghrelin levels tested in the study
View evidence from paper
“increasing ghrelin levels, through subcutaneous injections or calorie restriction, produced anxiolytic- and antidepressant-like responses”
Calorie Restriction Treatment
Calorie restriction was applied as an alternative method to increase ghrelin levels
Note: This is the second method of increasing ghrelin levels tested in the study
View evidence from paper
“increasing ghrelin levels, through subcutaneous injections or calorie restriction, produced anxiolytic- and antidepressant-like responses”
Elevated Plus Maze Testing
Mice were tested in the elevated plus maze to assess anxiety-like behavior following ghrelin administration or calorie restriction
Note: Behavioral testing was assisted by study personnel
View evidence from paper
“produced anxiolytic- and antidepressant-like responses in the elevated plus maze and forced swim test”