Elevated Plus Maze
Objective: Assessment of anxiety-like behavior in response to ghrelin administration or calorie restriction using the elevated plus maze test
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Protocol Steps
Ghrelin Administration via Subcutaneous Injection
Ghrelin was administered to mice through subcutaneous injections to increase ghrelin levels
Note: This is one method of increasing ghrelin levels tested in the study
View evidence from paper
“increasing ghrelin levels, through subcutaneous injections or calorie restriction, produced anxiolytic- and antidepressant-like responses”
Calorie Restriction Treatment
Calorie restriction was applied as an alternative method to increase ghrelin levels
Note: This is the second method of increasing ghrelin levels tested in the study
View evidence from paper
“increasing ghrelin levels, through subcutaneous injections or calorie restriction, produced anxiolytic- and antidepressant-like responses”
Elevated Plus Maze Testing
Mice were tested in the elevated plus maze to assess anxiety-like behavior following ghrelin administration or calorie restriction
Note: Behavioral testing was assisted by study personnel
View evidence from paper
“produced anxiolytic- and antidepressant-like responses in the elevated plus maze and forced swim test”