Elevated Plus Maze
Objective: Measure anxiety-like behavior by assessing exploration of open versus closed arms in mice subjected to chronic social defeat stress
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Equipment1
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Protocol Steps
Chronic Social Defeat Stress (CSDS) Model Implementation
Establish depression model in mice through chronic social defeat stress protocol
Note: This is the baseline stress condition for all subsequent behavioral tests
View evidence from paper
“A chronic social defeat stress (CSDS) depression model was performed to explore whether EDA could produce antidepressant effects”
Inhibitor Administration (if applicable)
Inject EX527 (Sirt1 inhibitor) and ML385 (Nrf2 inhibitor) intraperitoneally 30 minutes before EDA injection daily
Note: Intraperitoneal injection route; timing is critical - 30 minutes prior to EDA
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
EDA Treatment Administration
Administer EDA daily following inhibitor injection (if applicable)
Note: Administered after 30-minute interval from inhibitor injection
View evidence from paper
“EX527, a Sirt1 inhibitor and ML385, a Nrf2 inhibitor were injected intraperitoneally 30 min before EDA injection daily”
Elevated Plus Maze Test
Place mouse in elevated plus maze apparatus and measure anxiety-like behavior through assessment of exploration patterns in open versus closed arms
Note: One of multiple behavioral tests performed to assess depressive, anxiety-like and cognitive behaviors
View evidence from paper
“Behaviors tests were carried out to examine depressive, anxiety-like and cognitive behaviors including social interaction (SI) test, sucrose preference test (SPT), open field test (OFT), elevated plus maze (EPM)”
AAV Vector Infusion (for Gpx4 knockdown experiments)
Perform adeno-associated virus vector containing miRNAi (Gpx4)–EGFP infusion to determine effects of Gpx4 on CSDS mice with EDA treatment
Note: Knockdown experiments performed as separate experimental condition
View evidence from paper
“Knockdown experiments were performed to determine the effects of Gpx4 on CSDS mice with EDA treatment by an adeno-associated virus (AAV) vector containing miRNAi (Gpx4)–EGFP infusion”