Source Paper
Cerebellar neurocan gene expression is a common pathogenic factor of trigeminal neuralgia and depression
Zeng Z, Jiang J, Wu X, Liao X, Wang Y et al.
J Headache Pain • 2025
Elevated Plus Maze Test
Objective: To assess emotional behavior and depressive-like behavior in animals using anxiety measurements
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Protocol Steps
Equipment setup
Set up the elevated plus maze apparatus elevated 50 cm above the ground
View evidence from paper
“The entire apparatus was elevated 50 cm above the ground”
Test timing
Conduct measurements on the 28th day post-surgery for both groups
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“Measurements were conducted on the 28th day post-surgery for both the Sham and TN groups”
Animal placement
Place rats in the center of the maze facing the open arms
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“Rats were placed in the center of the maze facing the open arms”
Data recording
Record the number of entries into open arms and duration of time spent in open arms within a 5-minute period using behavior analysis software
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“the number of entries into the open arms and the duration of time spent in the open arms within a 5-minute period were recorded”
Data calculation
Calculate the percentages of time spent in the open arms
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“Subsequently, the percentages of time spent in the open arms were calculated”
Equipment cleaning
Clean the device with low-concentration alcohol and thoroughly dry before the next experiment
View evidence from paper
“The device was cleaned with low-concentration alcohol, and thoroughly dried prior to the next experiment”