Source Paper
Cerebellar neurocan gene expression is a common pathogenic factor of trigeminal neuralgia and depression
Zeng Z, Jiang J, Wu X, Liao X, Wang Y et al.
J Headache Pain • 2025
Elevated Plus Maze Test
Objective: To assess emotional behavior and depressive-like behavior in animals using anxiety measurements
This is a Elevated Plus Maze Test protocol using Sprague-Dawley rats as the model organism. The procedure involves 6 procedural steps, 2 equipment items, 1 materials. Extracted from a 2025 paper published in J Headache Pain.
Model and subjects
Sprague-Dawley rats • SD • male • adult • 12
Study window
Estimated timing pending
Core workflow
Equipment setup • Test timing • Animal placement
Primary readouts
- Number of entries into open arms
- Duration of time spent in open arms
- Percentage of time spent in open arms
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Equipment setup
Set up the elevated plus maze apparatus elevated 50 cm above the ground
View evidence from paper
“The entire apparatus was elevated 50 cm above the ground”
Test timing
Conduct measurements on the 28th day post-surgery for both groups
View evidence from paper
“Measurements were conducted on the 28th day post-surgery for both the Sham and TN groups”
Animal placement
Place rats in the center of the maze facing the open arms
View evidence from paper
“Rats were placed in the center of the maze facing the open arms”
Data recording
Record the number of entries into open arms and duration of time spent in open arms within a 5-minute period using behavior analysis software
View evidence from paper
“the number of entries into the open arms and the duration of time spent in the open arms within a 5-minute period were recorded”
Data calculation
Calculate the percentages of time spent in the open arms
View evidence from paper
“Subsequently, the percentages of time spent in the open arms were calculated”
Equipment cleaning
Clean the device with low-concentration alcohol and thoroughly dry before the next experiment
View evidence from paper
“The device was cleaned with low-concentration alcohol, and thoroughly dried prior to the next experiment”