Source Paper
Cerebellar neurocan gene expression is a common pathogenic factor of trigeminal neuralgia and depression
Zeng Z, Jiang J, Wu X, Liao X, Wang Y et al.
J Headache Pain • 2025
Von Frey Filament Test
Objective: Assessment of mechanical allodynia behavior in the rat orofacial region
This is a Von Frey Filament Test protocol using Sprague-Dawley rats as the model organism. The procedure involves 9 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in J Headache Pain.
Model and subjects
Sprague-Dawley rats • SD • male • adult • 12
Study window
~3 day study window | ~30 minutes hands-on
Core workflow
Baseline measurements • Schedule assessments • Prepare animal
Primary readouts
- Behavioral reactions to mechanical stimulation
- Positive response threshold (filament scale)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Baseline measurements
Obtain baseline measurements through continuous testing over 3 days prior to surgery
View evidence from paper
“Baseline measurements were obtained through continuous testing over a 3-day period prior to surgery”
Schedule assessments
Conduct assessments on specific days post-surgery
View evidence from paper
“Assessments were conducted on the day preceding the surgery and at 7, 14, 21, and 28 days post-surgery”
Prepare animal
Shave facial fur and prepare skin one day prior to behavioral test
View evidence from paper
“Facial fur was shaved, and the skin was prepared one day prior to each behavioral test”
Acclimatization
Allow animals to acclimatize to testing environment for 30 minutes
View evidence from paper
“Animals were acclimatized to the testing environment for 30 minutes on each experimental day”
Begin testing
Start testing using von Frey filaments with lowest pressure
View evidence from paper
“Testing was initiated using the von Frey filaments with the lowest pressure”
Apply filaments
Test each filament five times with 30-second intervals between stimuli
View evidence from paper
“Each filament was tested five times with a 30-second inter-stimulus interval”
Record responses
Record responses to mechanical stimulation
View evidence from paper
“responses to mechanical stimulation were recorded”
Define positive response
Document positive response as three or more behavioral reactions out of five trials
View evidence from paper
“A positive response was defined as three or more behavioral reactions out of five trials”
Repeat testing
Perform three consecutive behavioral tests with at least 30 seconds between each
View evidence from paper
“All animals underwent three consecutive behavioral tests with at least 30 seconds between each”