Source Paper
Cerebellar neurocan gene expression is a common pathogenic factor of trigeminal neuralgia and depression
Zeng Z, Jiang J, Wu X, Liao X, Wang Y et al.
J Headache Pain • 2025
Von Frey Filament Test
Objective: Assessment of mechanical allodynia behavior in the rat orofacial region
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Protocol Steps
Baseline measurements
Obtain baseline measurements through continuous testing over 3 days prior to surgery
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“Baseline measurements were obtained through continuous testing over a 3-day period prior to surgery”
Schedule assessments
Conduct assessments on specific days post-surgery
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“Assessments were conducted on the day preceding the surgery and at 7, 14, 21, and 28 days post-surgery”
Prepare animal
Shave facial fur and prepare skin one day prior to behavioral test
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“Facial fur was shaved, and the skin was prepared one day prior to each behavioral test”
Acclimatization
Allow animals to acclimatize to testing environment for 30 minutes
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“Animals were acclimatized to the testing environment for 30 minutes on each experimental day”
Apply filaments
Test each filament five times with 30-second intervals between stimuli
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“Each filament was tested five times with a 30-second inter-stimulus interval”
Record responses
Record responses to mechanical stimulation
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“responses to mechanical stimulation were recorded”
Define positive response
Document positive response as three or more behavioral reactions out of five trials
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“A positive response was defined as three or more behavioral reactions out of five trials”
Repeat testing
Perform three consecutive behavioral tests with at least 30 seconds between each
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“All animals underwent three consecutive behavioral tests with at least 30 seconds between each”