Source Paper
Cerebellar neurocan gene expression is a common pathogenic factor of trigeminal neuralgia and depression
Zeng Z, Jiang J, Wu X, Liao X, Wang Y et al.
J Headache Pain • 2025
Open Field Test
behavioralSprague-Dawley ratsSD
Objective: Assess the emotional behavior of animals
Protocol Steps
1
Timing
Conduct test on the 28th day post-surgery
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“All animals were assessed on the 28th day post-surgery”
2
Environment preparation
Perform experiment in quiet setting with dim lighting
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“The experiment was performed in a quiet setting with dim lighting”
3
Animal acclimatization
Place rats in behavioral laboratory for one hour adaptation
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“rats were placed in the behavioral laboratory for one hour for adaptation”
4
Animal placement
Gently place each rat in the central area of the open box
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“Each rat was gently placed in the central area of the open box”
5
Free movement period
Allow rat to move freely for 5 minutes
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“allowed to move freely for 5 min”
6
Equipment cleaning
Clean box with low-concentration alcohol after each trial
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“the box was promptly cleaned with low-concentration alcohol”
7
Wait for next trial
Begin subsequent trials only after alcohol fully evaporated
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“Subsequent trials commenced only after the alcohol had fully evaporated”