Source Paper
Cerebellar neurocan gene expression is a common pathogenic factor of trigeminal neuralgia and depression
Zeng Z, Jiang J, Wu X, Liao X, Wang Y et al.
J Headache Pain • 2025
Open Field Test
Objective: Assess the emotional behavior of animals
This is a Open Field Test protocol using Sprague-Dawley rats as the model organism. The procedure involves 7 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in J Headache Pain.
Model and subjects
Sprague-Dawley rats • SD • male • adult • 12
Study window
~5 minutes hands-on
Core workflow
Timing • Environment preparation • Animal acclimatization
Primary readouts
- total movement distance
- movement speed
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Timing
Conduct test on the 28th day post-surgery
View evidence from paper
“All animals were assessed on the 28th day post-surgery”
Environment preparation
Perform experiment in quiet setting with dim lighting
View evidence from paper
“The experiment was performed in a quiet setting with dim lighting”
Animal acclimatization
Place rats in behavioral laboratory for one hour adaptation
View evidence from paper
“rats were placed in the behavioral laboratory for one hour for adaptation”
Animal placement
Gently place each rat in the central area of the open box
View evidence from paper
“Each rat was gently placed in the central area of the open box”
Free movement period
Allow rat to move freely for 5 minutes
View evidence from paper
“allowed to move freely for 5 min”
Equipment cleaning
Clean box with low-concentration alcohol after each trial
View evidence from paper
“the box was promptly cleaned with low-concentration alcohol”
Wait for next trial
Begin subsequent trials only after alcohol fully evaporated
View evidence from paper
“Subsequent trials commenced only after the alcohol had fully evaporated”