Energy Intake Measurement
Objective: Measurement of energy intake in lean mice, diet-induced obesity mice, and ob/ob obese mice after GHS-R antagonist administration
This is a Energy Intake Measurement protocol using mouse as the model organism. The procedure involves 6 procedural steps, 4 equipment items, 3 materials. Extracted from a 2003 paper published in Gut.
Model and subjects
mouse • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Administer GHS-R antagonist • Measure energy intake • Measure gastric emptying rate
Primary readouts
- Energy intake (food consumption)
- Gastric emptying rate
- Body weight gain
- Glycaemic control (glucose levels)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Administer GHS-R antagonist
Administer GHS-R antagonist to lean mice, diet-induced obesity mice, and ob/ob obese mice
Note: For repeated administration studies, continued for six days in ob/ob obese mice
View evidence from paper
“Energy intake and gastric emptying were measured after administration of GHS-R antagonists”
Measure energy intake
Measure food consumption and energy intake in all three mouse groups following GHS-R antagonist administration
Note: GHS-R antagonists decreased energy intake in lean mice, diet-induced obesity mice, and ob/ob obese mice
View evidence from paper
“GHS-R antagonists decreased energy intake in lean mice, in mice with diet induced obesity, and in ob/ob obese mice”
Measure gastric emptying rate
Assess the rate of gastric emptying following GHS-R antagonist administration
Note: GHS-R antagonists reduced the rate of gastric emptying
View evidence from paper
“it also reduced the rate of gastric emptying”
Repeated administration protocol for ob/ob mice
Continue GHS-R antagonist administration for six days in ob/ob obese mice
Note: Repeated administration decreased body weight gain and improved glycaemic control
View evidence from paper
“Repeated administration of GHS-R antagonist was continued for six days in ob/ob obese mice”
Measure body weight gain
Monitor body weight changes in ob/ob obese mice following repeated GHS-R antagonist administration
Note: Repeated administration decreased body weight gain
View evidence from paper
“Repeated administration of GHS-R antagonist decreased body weight gain”
Assess glycaemic control
Measure glucose levels and glycaemic control in ob/ob obese mice after repeated GHS-R antagonist administration
Note: Repeated administration improved glycaemic control in ob/ob obese mice
View evidence from paper
“Repeated administration of GHS-R antagonist decreased body weight gain and improved glycaemic control in ob/ob obese mice”