Excisional Wound Closure Assessment
Objective: Determine the requirement for inducible nitric oxide synthase (iNOS) in excisional wound closure by comparing wound closure rates in iNOS knockout mice versus wild-type controls
This is a Excisional Wound Closure Assessment protocol using mouse as the model organism. The procedure involves 5 procedural steps, 2 materials. Extracted from a 1998 paper published in Journal of Clinical Investigation.
Model and subjects
mouse • iNOS knockout and wild-type • unknown • Not specified • Not specified
Study window
~6 day study window
Core workflow
Create excisional wounds • Measure wound closure in iNOS knockout versus wild-type mice • Administer iNOS inhibitor to wild-type mice
Primary readouts
- Wound closure rate (percentage delay in closure)
- iNOS mRNA expression levels and timing
- Human iNOS expression in adenoviral vector-treated animals
- Reversal of delayed wound healing phenotype
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Create excisional wounds
Excisional wounds were created in iNOS knockout mice and wild-type control animals
Note: Baseline wound creation for comparison between genotypes
View evidence from paper
“Experiments were carried out to determine the requirement for iNOS in closing excisional wounds”
Measure wound closure in iNOS knockout versus wild-type mice
Wound closure was measured and compared between iNOS knockout mice and wild-type animals
Note: Wound closure was delayed by 31% in iNOS knockout mice compared with wild-type animals
View evidence from paper
“Wound closure was delayed by 31% in iNOS knockout mice compared with wild-type animals”
Administer iNOS inhibitor to wild-type mice
Wild-type mice were given continuous infusion of N6-(iminoethyl)-L-lysine, a partially selective iNOS inhibitor
Note: This treatment produced an identical delay in wound closure as observed in iNOS knockout mice
View evidence from paper
“An identical delay in wound closure was observed in wild-type mice given a continuous infusion of the partially selective iNOS inhibitor N6-(iminoethyl)-L-lysine”
Apply adenoviral iNOS vector to iNOS-deficient mice
A single application of adenoviral vector containing human iNOS cDNA (AdiNOS) was applied at the time of wounding
Note: This treatment completely reversed the delayed wound healing phenotype in iNOS-deficient mice
View evidence from paper
“Delayed wound healing in iNOS-deficient mice was completely reversed by a single application of an adenoviral vector containing human iNOS cDNA (AdiNOS) at the time of wounding”
Measure iNOS mRNA expression by reverse transcription PCR
Reverse transcription PCR was performed to identify iNOS mRNA expression in wild-type mice and confirm human iNOS expression in treated animals
Note: iNOS mRNA expression peaked 4-6 days after wounding in wild-type mice; human iNOS expression was confirmed in AdiNOS-treated animals
View evidence from paper
“Reverse transcription PCR identified iNOS mRNA expression in wild-type mice peaking 4-6 d after wounding, and confirmed expression of human iNOS in the adenoviral vector containing human iNOS cDNA-treated animals”