Fasting Experiment
Objective: To examine metabolic changes in mice following 24-hour food deprivation
This is a Fasting Experiment protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 4 materials. Extracted from a 2009 paper published in Aging.
Model and subjects
mouse • C57BL/6 • male • 3 months old
Study window
~24 hours hands-on
Core workflow
Animal preparation and housing • Food removal • Tissue collection
Primary readouts
- SIRT3 gene expression
- Metabolic changes following fasting
- Tissue-specific protein expression
Key equipment and reagents
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Protocol Steps
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Animal preparation and housing
C57BL/6 male mice at 3 months of age were singly housed in individual cages
Note: Mice were 3 months old at start of fasting experiment
View evidence from paper
“For the fasting experiment, food was removed from 3 months old C57BL/6 male mice”
Food removal
Food was removed from mice at 6pm for a 24-hour period
Note: Fasting period started at 6pm
View evidence from paper
“food was removed from 3 months old C57BL/6 male mice at 6pm for 24 hours”
Tissue collection
Various tissues were harvested from mice following the 24-hour fasting period for analysis
Note: Tissues were collected for examination of SIRT3 gene expression and other metabolic markers
View evidence from paper
“Various tissues were also harvested from mice fed the control diet to examine SIRT3 gene expression”