Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle

Orsolya M. Palacios, Juan J. Carmona, Shaday Michan, Ke Yun Chen, Yasuko Manabe et al.

Aging • 2009

Fasting Experiment

behavioralmouseC57BL/6

Objective: To examine metabolic changes in mice following 24-hour food deprivation

Materials & Equipment Checklist

6 items

Gather these items before starting the experiment. Check off items as you prepare.

Rodent running wheels

Nalgene

Individual cages

NIH-31 standard diet

Harlan Teklad

NIH-31/NIA-fortified diet

Harlan Teklad

35% fat-enriched chow

BioServ

PicoLab Mouse Diet 20

LabDiet/Purina

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C57BL/6 male mice at 3 months of age were singly housed in individual cages

Note: Mice were 3 months old at start of fasting experiment

View evidence from paper

“For the fasting experiment, food was removed from 3 months old C57BL/6 male mice”

Food was removed from mice at 6pm for a 24-hour period

24 hours

Note: Fasting period started at 6pm

View evidence from paper

“food was removed from 3 months old C57BL/6 male mice at 6pm for 24 hours”

Various tissues were harvested from mice following the 24-hour fasting period for analysis

Note: Tissues were collected for examination of SIRT3 gene expression and other metabolic markers

View evidence from paper

“Various tissues were also harvested from mice fed the control diet to examine SIRT3 gene expression”