Source Paper
No effect of partial reinforcement on fear extinction learning and memory in C57BL/6J mice
Su CJ, Fukunaga Y, Penna S, Cazares VA
Learn Mem • 2025
Fear Extinction Training
Objective: To assess fear extinction learning and memory by measuring freezing behavior during unreinforced conditioned stimulus presentations
Gather these items before starting the experiment. Check off items as you prepare.
Equipment5
1 item available from ConductScience
Estimated: $5,900.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Fear conditioning training
Conduct delayed fear conditioning in context A with tone-shock pairings over 1-2 days
View evidence from paper
“All experiments consisted of one or 2 days of delayed FC”
Context preparation for extinction
Switch to context B with different environmental cues for extinction training
View evidence from paper
“FE and the 4 CS tone-tests were conducted in context B”
Fear extinction training
Present 12 unreinforced conditioned stimuli per day for 1-3 consecutive days
View evidence from paper
“mice received between one and three consecutive days of FE training, each day of which consisted of 12 unreinforced exposures to the CS”
Recent extinction memory test
Test extinction memory with 4 conditioned stimuli at 2 days after extinction training
View evidence from paper
“mice were exposed to four CS at 2 and 30 days after extinction training”
Remote extinction memory test
Test long-term extinction memory with 4 conditioned stimuli at 30 days after extinction training
View evidence from paper
“mice were exposed to four CS at 2 and 30 days after extinction training”