Source Paper
Atrophin-1 antisense oligonucleotide provides robust protection from pathology in a fully humanized DRPLA model
Smith VL, Gidi BZ, Bragg RM, Cantle JP, Ben-Varon A et al.
Mol Ther Nucleic Acids • 2025
Fixed Rotarod
Objective: Motor coordination assessment using fixed rotarod apparatus
This is a Fixed Rotarod protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 equipment items. Extracted from a 2025 paper published in Mol Ther Nucleic Acids.
Model and subjects
mouse • not specified • male and female • 6 to 7 weeks • not specified
Study window
~7 week study window | ~5 minutes hands-on
Core workflow
Training phase • Testing phase • Age timing
Primary readouts
- Motor coordination performance on rotarod
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Training phase
Each mouse underwent three 5-minute training trials at two different speeds
View evidence from paper
“Each mouse went through three 5-min training trials at two speeds (4 and 8 rpm)”
Testing phase
Mice were tested for three trials at both training speeds with maximum trial duration of 5 minutes
View evidence from paper
“Mice were then tested for three trials at both speeds with a maximum time of 5 min”
Age timing
All training and testing conducted when mice were 6-7 weeks old
View evidence from paper
“All mice were tested and trained from 6 to 7 weeks of age”