Fluid Percussion Injury
Objective: To induce diffuse traumatic brain injury in male mice using midline fluid percussion injury (FPI) and assess cortical neuropathology and inflammation at acute, subacute, and chronic timepoints
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Protocol Steps
Microglial depletion
Administer PLX5622, a CSF1R antagonist, to deplete microglia before injury induction
Note: Depletion occurs prior to FPI procedure
View evidence from paper
“Microglia were depleted with PLX5622, a CSF1R antagonist, before midline fluid percussion injury (FPI)”
Midline fluid percussion injury
Perform midline fluid percussion injury to induce diffuse traumatic brain injury in male mice
Note: Injury is delivered to the midline of the brain
View evidence from paper
“midline fluid percussion injury (FPI) was performed to induce diffuse traumatic brain injury in male mice”
Assessment at acute timepoint
Assess cortical neuropathology and inflammation at 1 day postinjury (dpi) using neuropathology mRNA panel
Note: Acute timepoint for gene expression analysis
View evidence from paper
“cortical neuropathology/inflammation was assessed using a neuropathology mRNA panel at acute [1 d postinjury (dpi)]”
Assessment at subacute timepoint
Assess cortical neuropathology and inflammation at 7 days postinjury using neuropathology mRNA panel and single-cell RNA sequencing
Note: Critical timepoint in evolution from acute to chronic pathogenesis; single-cell RNA sequencing completed at this timepoint
View evidence from paper
“single-cell RNA sequencing was completed 7 dpi, a critical time point in the evolution from acute to chronic pathogenesis”
Assessment at chronic timepoint
Assess cortical neuropathology and inflammation at 30 days postinjury; measure dendritic complexity, neuronal connectivity, and cognitive function
Note: Chronic timepoint; includes behavioral and structural assessments
View evidence from paper
“reduced cortical dendritic complexity 7 dpi, reduced neuronal connectively 30 dpi, and cognitive impairment 30 dpi”